Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain BL21(DE3) | Sulfurisphaera tokodaii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, 5-8 mg/ml in 20 mM TrisHCl buffer, pH 7.0, hanging-drop vapour-diffusion method, 26°C, from 0.002 ml protein solution is mixed with 0.002 ml of reservoir solution containing 100 mM sodium HEPES buffer, pH 7.5, containing 100 mM NaCl and 1.6 M (NH4)2SO4, equilibration against 0.7 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49074 | - |
x * 49074, sequence calculation | Sulfurisphaera tokodaii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsn | Sulfurisphaera tokodaii | dual activity of the ND-AspRS since an asparaginyl-tRNA synthetase is missing in Sulfolobus tokodaii strain 7 | AMP + diphosphate + aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + tRNAAsp | Sulfurisphaera tokodaii | - |
AMP + diphosphate + aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Sulfolobus tokodaii | - |
- |
- |
no activity in Sulfolobus tokodaii 7 | - |
- |
- |
Sulfurisphaera tokodaii | - |
a crenarchaeon, strain 7, gene ST0205 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydroxyapatite chromatography | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsn | dual activity of the ND-AspRS since an asparaginyl-tRNA synthetase is missing in Sulfolobus tokodaii strain 7 | Sulfurisphaera tokodaii | AMP + diphosphate + aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Sulfurisphaera tokodaii | AMP + diphosphate + aspartyl-tRNAAsp | - |
? | |
additional information | the purified protein has the ability to catalyze the aspartylation of hydroxylamine through an aspartyl-AMP intermediate | Sulfurisphaera tokodaii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49074, sequence calculation | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
nondiscriminating aspartyl-tRNA synthetase | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
- |
Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sulfurisphaera tokodaii |