6.1.1.21: histidine-tRNA ligase

This is an abbreviated version!
For detailed information about histidine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.21

6.1.1.21

synthetases

aminoacylation

aminoacyl-trna

autoantibody

histidylation

myositis

anti-jo-1

polymyositis

anticodon

dermatomyositis

aarss

perrault

hiss

histidinol

anti-synthetase

guanylyltransferase

hsd17b4

myositis-specific

c10orf2

thrrs

threonyl-trna

trna-like

acid-starved

seryl-trna

analysis

medicine

Reaction

Synonyms

antihistidyl-tRNA synthetase, class II histidyl-tRNA synthetase, HARS, HARS1, HARS2, HisRS, HisRS-1, HisRS-2, Histidine translase, Histidine--tRNA ligase, Histidine--tRNA ligase homolog, histidine-tRNA ligase, histidine-tRNA ligase homolog, histidyl tRNA synthetase, Histidyl-transfer ribonucleate synthetase, histidyl-transfer RNA synthetase, Histidyl-tRNA synthetase, HRS, HTS1, Jo-1, Jo-1 antigen, mitochondrial histidyl tRNA synthetase, Synthetase, histidyl-transfer ribonucleate

ECTree

6 Ligases

6.1 Forming carbon-oxygen bonds

6.1.1 Ligases forming aminoacyl-tRNA and related compounds

6.1.1.21 histidine-tRNA ligase

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Results	in table
2	Activating Compound
49530	AA Sequence
4	Application
1	CAS Registry Number
22	Cloned(Commentary)
36	Cofactor
13	Crystallization (Commentary)
260	Disease/ Diagnostics
1	Expression
14	General Information
12	General Stability
7	IC50 Value
39	Inhibitors
14	kcat/KM [mM/s]
78	KM Value [mM]
22	Localization
19	Metals/Ions
41	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
111	Organism
1	Oxidation Stability
4	Pathway
86	PDB ID
12	pH Optimum
2	pH Range
1	Posttranslational Modification
57	Protein Variants
26	Purification (Commentary)
12	Reaction
62	Reaction Type
101	Reference
1	Renatured (Commentary)
29	Source Tissue
9	Specific Activity [micromol/min/mg]
5	Storage Stability
134	Substrates and Products (Substrate)
36	Subunits
346	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
61	Turnover Number [1/s]

Purification

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Purification on EC 6.1.1.21 - histidine-tRNA ligase

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5110fold

800fold to 95% purity

Salmonella enterica subsp. enterica serovar Typhimurium

O52765

650438

by a Ni-NTA affinity column followed by a XK26/60 Superdex 75 column

2 entries

employing Q-Sepharose and hydroxyapatite chromatography, by utilizing the His-tag

Escherichia coli

P60906

704512

native HisRS and fusion HisRS

Streptococcus dysgalactiae subsp. equisimilis

357

Ni-NTA column chromatography

3 entries

Ni-NTA column chromatography and Superdex 200 gel filtration

2 entries

Ni-NTA column chromatography, and Superdex 200 gel filtration

Homo sapiens

P12081

744439

Ni-NTA column chromatography, Mono S cation-exchange column chromatography, and Superdex 200 gel filtration

partial

378

partially 351fold, fully 820fold purified, recombinant from overexpression in large scale

Escherichia coli

P60906

650438

recombinant from Escherichia coli

Staphylococcus aureus

P60911

649862

recombinant maltose-binding fusion HisRS active site fragments, HisRS1-HisRS4, from Escherichia coli strain BL21(DE3)pLysS by amylose affinity chromatography, fusion protein cleavage by TEV. The fusion protein does not affect urzyme activity

Escherichia coli

715579

recombinant wild-type enzyme and mutants

Escherichia coli

650256