Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.21 extracted from

  • Chen, C.C.; Somberg, E.W.
    Purification and characterization of histidyl-transfer RNA synthetase from Neurospora crassa (1980), Biochim. Biophys. Acta, 613, 514-525.
    View publication on PubMed

General Stability

General Stability Organism
bovine serum albumin required to prevent inactivation Neurospora crassa
loss of activity after freezing and thawing Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
Argininosuccinic acid poor inhibitor Neurospora crassa
Imidazoleglycerol phosphate
-
Neurospora crassa
Indoleglycerol phosphate
-
Neurospora crassa
L-histidinol
-
Neurospora crassa
ornithine poor inhibitor Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
tRNAHis aminoacylation Neurospora crassa
0.0058
-
His aminoacylation Neurospora crassa
0.59
-
ATP aminoacylation Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration is 20 mM Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62500
-
2 * 62500, SDS-PAGE Neurospora crassa
125000
-
gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Neurospora crassa

Storage Stability

Storage Stability Organism
-20°C, 10 mM Hepes, pH 7.4, 1 mM magnesium acetate, 1 mM KCl, 0.5 mM DTT, 0.1 mM phenylmethylsulfonyl fluoride, 50% glycerol, stable for 6 months Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-histidine + tRNAHis
-
Neurospora crassa AMP + diphosphate + L-histidyl-tRNAHis
-
?

Subunits

Subunits Comment Organism
dimer 2 * 62500, SDS-PAGE Neurospora crassa