Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.1.1.21: histidine-tRNA ligase

This is an abbreviated version!
For detailed information about histidine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.21

Reaction

ATP
+
L-histidine
+
tRNAHis
=
AMP
+
diphosphate
+
L-histidyl-tRNAHis

Synonyms

antihistidyl-tRNA synthetase, class II histidyl-tRNA synthetase, HARS, HARS1, HARS2, HisRS, HisRS-1, HisRS-2, Histidine translase, Histidine--tRNA ligase, Histidine--tRNA ligase homolog, histidine-tRNA ligase, histidine-tRNA ligase homolog, histidyl tRNA synthetase, Histidyl-transfer ribonucleate synthetase, histidyl-transfer RNA synthetase, Histidyl-tRNA synthetase, HRS, HTS1, Jo-1, Jo-1 antigen, mitochondrial histidyl tRNA synthetase, Synthetase, histidyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.21 histidine-tRNA ligase

Cloned

Cloned on EC 6.1.1.21 - histidine-tRNA ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
determination of DNA sequence, genomic organization, and chromosome mapping to 5q31.3
DNA and amino acid sequence determination and analysis of HisRS gene variants, HisRS genes, which map closely to the Egr1 locus, have conserved the 129/Sv haplotype despite numerous back-crossing of the null mice progeny with C57Bl/6J animals, genetic analysis of isozymes of HisRS encoded by the 129/Sv haplotype, allelic difference for mortalin and HisRS between 129/Sv and C57Bl/6J strains, overview
-
DNA sequence determination and analysis, phylogenetic development
DNA sequence determination and expression in strain JM105 of wild-type and mutant enzymes
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli MEOV1 cells
-
expression as enzyme-green fluorescent protein or as green fluorescent protein-enzyme construct
-
expression in Cos-1 cells
-
expression of C-terminally epitope-tagged wild-type and mutant HARS2 in 293T cells mitochondria
-
gene hisS, expression of HisRS active site fragments, HisRS1-HisRS4, as maltose-binding proteins fusion proteins in Escherichia coli strain BL21(DE3)pLysS
-
HTS1, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)
-
overexpression
overexpression in Escherichia coli DH5alpha
overexpression in Escherichia coli of a fusion HisRS, with additional 15 amino acids between the initiator Met and the second amino acid Lys
-
overexpression in Escherichia coli, as a fusion protein containing an additional 15 amino acids
-
recombinant HisRS allows complete histidylation of a Caulobacter crescentus tRNAHis transcript lacking G-1, addition of G-1 does not improve the aminoacylation
-
the full-length and a truncated variant is cloned into the Escherichia coli expression vector AVA0421 derived from vector pET14b
the vectors pWY30 anf pQE30 are used