5.4.99.B22: multisite-specific tRNA pseudouridine synthase

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For detailed information about multisite-specific tRNA pseudouridine synthase, go to the full flat file.

Reaction

Synonyms

cePus1p, dmPus1p, hPus1p, mPus1p, pseudouridine synthase 1, pseudouridine synthase Pus10, PUS1, Pus1p, Pus4, PUS6, PUS7, RluE, RNA:pseudouridine synthases 1, RPUSD4, scPus1p, spPus1p, SVR1, TgPUS1, xtPus1p, YmfC

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.99 Transferring other groups

                5.4.99.B22 multisite-specific tRNA pseudouridine synthase

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Results	in table
15	AA Sequence
1	Application
13	Cloned(Commentary)
2	Crystallization (Commentary)
21	Disease/ Diagnostics
48	General Information
2	Inhibitors
2	kcat/KM [mM/s]
4	KM Value [mM]
3	Localization
2	Metals/Ions
15	Natural Substrates/ Products (Substrates)
109	Organism
2	pH Optimum
24	Protein Variants
11	Purification (Commentary)
18	Reaction
33	Reference
7	Source Tissue
67	Substrates and Products (Substrate)
2	Subunits
51	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Turnover Number [1/s]

Crystallization

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Crystallization on EC 5.4.99.B22 - multisite-specific tRNA pseudouridine synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant detagged truncated enzyme mutant . DELTAhPus1p and mutant D146A DELTAhPus1p, sitting drop technique, mixing of 7 mg/ml protein in 20 mM HEPES, pH 7.0, 150 mM NaCl, 5 mM MgCl2, and 5 mM TCEP, with an equal amount of reservoir solution containing for DELTAhPus1p crystals 28% PEG 3350/glycerol, 0.1 M Bicine/Tris, pH 8.5, 10% amino acids mixture (with 0.02 M sodium L-glutamate, 0.02 M DL-alanine, 0.02 M glycine, 0.02 M DL-lysine HCl, 0.02 M DL-serine), and for D146A DELTAhPus1p crystals 17% PEG 8000, and 0.1 M HEPES, pH 8.0, both at 4°C, for 2-3 days, X-ray diffraction structure determination and analysis at 2.0-2.7 A resolution, respectively, molecular replacement using Pus1p structure, PDB ID 4ITS, as a model

Homo sapiens

Q9Y606

749148

sitting drop technique. The crystal structure of the catalytic domain of hPus1p and the D146A mutant of the enzyme is determined at 2.0 A resolution, alone and in a complex with several molecules present during crystallisation

Homo sapiens

Q9Y606

749148