5.4.99.B22: multisite-specific tRNA pseudouridine synthase

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For detailed information about multisite-specific tRNA pseudouridine synthase, go to the full flat file.

Reaction

Synonyms

cePus1p, dmPus1p, hPus1p, mPus1p, pseudouridine synthase 1, pseudouridine synthase Pus10, PUS1, Pus1p, Pus4, PUS6, PUS7, RluE, RNA:pseudouridine synthases 1, RPUSD4, scPus1p, spPus1p, SVR1, TgPUS1, xtPus1p, YmfC

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.99 Transferring other groups

                5.4.99.B22 multisite-specific tRNA pseudouridine synthase

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Results	in table
15	AA Sequence
1	Application
13	Cloned(Commentary)
2	Crystallization (Commentary)
21	Disease/ Diagnostics
48	General Information
2	Inhibitors
2	kcat/KM [mM/s]
4	KM Value [mM]
3	Localization
2	Metals/Ions
15	Natural Substrates/ Products (Substrates)
109	Organism
2	pH Optimum
24	Protein Variants
11	Purification (Commentary)
18	Reaction
33	Reference
7	Source Tissue
67	Substrates and Products (Substrate)
2	Subunits
51	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Turnover Number [1/s]

Metals Ions

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Metals Ions on EC 5.4.99.B22 - multisite-specific tRNA pseudouridine synthase

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

KCl

Saccharomyces cerevisiae

Q12211

the complex of Pus1 with tRNA is sensitive to high ionic strength. The optimal concentration of KCl for Pus1 binding is 100 mM. Above 400 mM KCl, no complex is detected

5860

Zinc

Saccharomyces cerevisiae

Q12211

contains one atom of zinc per 63000 Da monomer. This zinc ion could not be removed by treatment with EDTA or urea. However, a zinc-depleted enzyme is obtained after prolonged dialysis against the specific chelating agent 1,10-phenanthroline. Removal of the zinc ion results in inactivation of the enzyme with concomitant loss of its ability to bind tRNA. Structural role for the zinc in the tRNA-pseudouridine synthetase Pus1

5856