5.4.99.B22: multisite-specific tRNA pseudouridine synthase
This is an abbreviated version!
For detailed information about multisite-specific tRNA pseudouridine synthase, go to the full flat file.
Word Map on EC 5.4.99.B22
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5.4.99.B22
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pseudouridylation
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anemia
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sideroblastic
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myopathy
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dyskerin
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anticodon
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mlasa
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spliceosomal
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nuclear-encoded
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agriculture
- 5.4.99.B22
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pseudouridylation
- anemia
-
sideroblastic
- myopathy
-
dyskerin
-
anticodon
-
mlasa
-
spliceosomal
-
nuclear-encoded
- agriculture
Reaction
Synonyms
cePus1p, dmPus1p, hPus1p, mPus1p, pseudouridine synthase 1, pseudouridine synthase Pus10, PUS1, Pus1p, Pus4, PUS6, PUS7, RluE, RNA:pseudouridine synthases 1, RPUSD4, scPus1p, spPus1p, SVR1, TgPUS1, xtPus1p, YmfC
ECTree
5.4.99.B22 multisite-specific tRNA pseudouridine synthaseAdvanced search results
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results (Engineering
Engineering on EC 5.4.99.B22 - multisite-specific tRNA pseudouridine synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D146A
site-directed mutagenesis of truncated enzyme mutant DELTAhPus1p, differences between structures of DELTAhPus1p and DELTAhPus1pD146A are limited to external, poorly conserved loops, which confirm the flexibility of these regions
R116A
the mutant exhibits some activity with the yeast pre-tRNAIle substrate. Low activity with the mouse tRNAMet substrate
R116C
the activity of the mutant enzyme with the yeast pre-tRNAIle substrate is approximately one-third that of the wild-type levels. No activity with the mouse tRNAMet substrate
R116E
the mutant is inactive with with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate
R116G
the mutant exhibits some activity with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate
R116H
the mutant is inactive with with the yeast pre-tRNAIle substrate. Low activity with the mouse tRNAMet substrate
R116K
the activity of the mutant enzyme with the yeast pre-tRNAIle substrate is approximately one-third that of the wild-type levels
R116N
the mutant exhibits some activity with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate
R116Q
the mutant exhibits some activity with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate
R116S
the mutant exhibits some activity with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate
R116W
the mutant is inactive with with the yeast pre-tRNAIle substrate. Low activity with the mouse tRNAMet substrate
Y173C
mutant enzyme retains some activity with yeast pre-tRNAIle. The activity is relatively low
Y173F
Y173G
mutant enzyme retains some activity with yeast pre-tRNAIle. The activity is relatively low
Y173T
additional information
Y173F
mutant enzyme retains some activity with yeast pre-tRNAIle. The activity is relatively low
Y173T
mutant enzyme retains some activity with yeast pre-tRNAIle. The activity is relatively low
additional information
generation of a truncated hPus1p version encompassing residues 83-394, i.e. DELTAhPus1p. The deleted N- and C-termini are not essential for either the RNA binding or the activity of the hPus1p enzyme
additional information
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generation of a truncated hPus1p version encompassing residues 83-394, i.e. DELTAhPus1p. The deleted N- and C-termini are not essential for either the RNA binding or the activity of the hPus1p enzyme
additional information
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generation of gene deletion mutant DELTApus1. which shows a U2 snRNA modification pattern identical to its parental wild-type strain ED666
additional information
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generation of gene deletion mutant DELTApus1. which shows a U2 snRNA modification pattern identical to its parental wild-type strain ED666
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additional information
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SNORA71 is expressed in an enzyme knockout mutant yeast strain, but do not restore pseudouridylation of the endogenous yeast U2 snRNA
