5.4.99.B22: multisite-specific tRNA pseudouridine synthase
This is an abbreviated version!
For detailed information about multisite-specific tRNA pseudouridine synthase, go to the full flat file.
Word Map on EC 5.4.99.B22
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5.4.99.B22
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pseudouridylation
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anemia
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sideroblastic
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myopathy
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dyskerin
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anticodon
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mlasa
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spliceosomal
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nuclear-encoded
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agriculture
- 5.4.99.B22
-
pseudouridylation
- anemia
-
sideroblastic
- myopathy
-
dyskerin
-
anticodon
-
mlasa
-
spliceosomal
-
nuclear-encoded
- agriculture
Reaction
Synonyms
cePus1p, dmPus1p, hPus1p, mPus1p, pseudouridine synthase 1, pseudouridine synthase Pus10, PUS1, Pus1p, Pus4, PUS6, PUS7, RluE, RNA:pseudouridine synthases 1, RPUSD4, scPus1p, spPus1p, SVR1, TgPUS1, xtPus1p, YmfC
ECTree
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Subunits
Subunits on EC 5.4.99.B22 - multisite-specific tRNA pseudouridine synthase
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additional information
the hPus1p enzyme binds to its RNA substrate as a monomer. The C-terminal end of hPus1p folds into two alpha-helices and these helices pack against the back of the hPus1p molecule, which prevents the dimerization observed in the bacterial homologue, TruA. This C-terminal region is responsible for the atypical RNA substrate binding and activity of hPus1p. Enzyme crystal structure analysis, overview
additional information
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the hPus1p enzyme binds to its RNA substrate as a monomer. The C-terminal end of hPus1p folds into two alpha-helices and these helices pack against the back of the hPus1p molecule, which prevents the dimerization observed in the bacterial homologue, TruA. This C-terminal region is responsible for the atypical RNA substrate binding and activity of hPus1p. Enzyme crystal structure analysis, overview
additional information
study of the oligomerisation in solution of purified recombinant yeast tRNA:pseudouridine synthetase Pus1 in the presence or absence oftRNA. The presence of the neutral detergent dodecyl-beta-D-maltoside helps in avoiding the oligomerization/inactivation of the purified Pus1 in the absence of tRNA