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5.4.99.B22: multisite-specific tRNA pseudouridine synthase

This is an abbreviated version!
For detailed information about multisite-specific tRNA pseudouridine synthase, go to the full flat file.

Word Map on EC 5.4.99.B22

Reaction

tRNA uridine26
=
tRNA pseudouridine26

Synonyms

cePus1p, dmPus1p, hPus1p, mPus1p, pseudouridine synthase 1, pseudouridine synthase Pus10, PUS1, Pus1p, Pus4, PUS6, PUS7, RluE, RNA:pseudouridine synthases 1, RPUSD4, scPus1p, spPus1p, SVR1, TgPUS1, xtPus1p, YmfC

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.B22 multisite-specific tRNA pseudouridine synthase

Cloned

Cloned on EC 5.4.99.B22 - multisite-specific tRNA pseudouridine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Saccharomyces cerevisiae
gene PUS1, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant Pus1, tagged with six histidine residues at its N-terminus is expressed in Escherichia coli
the PUS1 ORF is tagged at its amino-terminal end with six histidines and overexpressed in Escherichia coli
transcriptome-wide quantitative mapping of Psi