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5-fluoro-alpha-D-glucosyl fluoride
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behaves like a reversible inhibitor
acarbose
competitively inhibited by the potent alpha-glucosidase inhibitor acarbose, acarbose-binding site structure, overview
beta-mercaptoethanol
complete inhibition at 1 mM
D-gluconohydroximino-1,5-lactam
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D-glucose
in the presence of 10 mM D-glucose, TreS shows a 3.6fold increase in Km and a nearly unchanged Vmax for maltose, implying that D-glucose is a competitive inhibitor of TreS
dithiothreitol
9% inhibition at 5 mM, 12% at 10 mM
Fe3+
1 mM, 60% residual activity
Guanidine-HCl
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complete inhibition at 1 M, 51% inhibition of aggregated enzyme
Na+
90% residual activity at 1 mM
sodium dodecylsulfate
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1 mM, complete inhibition
sucrose
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competitive inhibition of the interconversion between maltose and trehalose
Urea
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90% inhibition at 2 M, 1% inhibition of aggregated enzyme
Al3+
98.5% inhibition at 1 mM
Al3+
complete inhibition at 2 mM
Al3+
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complete inhibition at 2 mM, 8% inhibition of aggregated enzyme
Al3+
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1 mM, complete inhibition
Ca2+
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93% residual activity at 5 mM
Ca2+
13.4% inhibition at 10 mM, 9.5% at 1 mM
Ca2+
about 10% residual activity at 10 mM
Ca2+
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the MTase activity of TreS is inhibited by 5 mM Ca2+ and other divalent cations
Ca2+
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the MTase activity of TreS is inhibited by 5 mM Ca2+ and other divalent cations
Ca2+
98% residual activity at 5 mM
Ca2+
WP_028494267
10 mM, 29% loss of activity
Co2+
1 mM, 38% loss of activity
Co2+
1 mM, 55% residual activity
Co2+
17.2% inhibition at 1 mM
Co2+
about 5% residual activity at 10 mM
Co2+
WP_028494267
1 mM, 56% loss of activity
Cu2+
1 mM, complete inhibition
Cu2+
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45.4% residual activity at 5 mM
Cu2+
1 mM, 5% residual activity
Cu2+
complete inhibition at 10 mM
Cu2+
52% residual activity at 1 mM
Cu2+
complete inhibition at 2 mM
Cu2+
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complete inhibition at 2 mM, 23% inhibition of aggregated enzyme
Cu2+
complete inhibition at 5 mM
Cu2+
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10 mM, 85% residual activity
Cu2+
34% inhibition at 1 mM, 41% at 10 mM
Cu2+
WP_028494267
1 mM, 83% loss of activity
Cu2+
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inhibition of recombinant fusion protein with N-terminal beta-amylase of Clostridium thermofluorogenes and C-terminal trehalose synthase or vice versa
EDTA
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68.5% residual activity at 5 mM
EDTA
inhibitory above 1 mM
EDTA
15% inhibition at 1 mM, 35% at 10 mM
EDTA
86% residual activity at 5 mM
EDTA
46% inhibition at 1 mM, 100% at 10 mM
Fe2+
92% residual activity at 1 mM
Fe2+
complete inhibition at 2 mM
Fe2+
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95% inhibition at 2 mM, 5% inhibition of aggregated enzyme
Hg2+
1 mM, 15% residual activity
Hg2+
complete inhibition at 1 mM
Hg2+
57% residual activity at 1 mM
Hg2+
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1 mM, complete inhibition
Hg2+
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inhibition of recombinant fusion protein with N-terminal beta-amylase of Clostridium thermofluorogenes and C-terminal trehalose synthase or vice versa
Mg2+
1 mM, 75% residual activity
Mg2+
8.5% inhibition at 1-10 mM
Mg2+
92% residual activity at 5 mM
Mg2+
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about 50% inhibition
Mn2+
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60.9% residual activity at 5 mM
Mn2+
20% inhibition at 1 mM, 30% at 10 mM
Mn2+
about 10% residual activity at 10 mM
Mn2+
86% residual activity at 5 mM
Mn2+
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10 mM, 60% residual activity
Ni2+
1 mM, 58% loss of activity
Ni2+
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49.4% residual activity at 5 mM
Ni2+
28.5% inhibition at 1mM, 45% at 10 mM
Ni2+
about 10% residual activity at 10 mM
Ni2+
66% inhibition at 1 mM, 75% at 10 mM
Pb2+
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Pb2+
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inhibition of recombinant fusion protein with N-terminal beta-amylase of Clostridium thermofluorogenes and C-terminal trehalose synthase or vice versa
SDS
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0.25% residual activity at 5 mM
SDS
95.1% inhibition at 5 mM
SDS
10% residual activity at 1 mM
SDS
complete inhibition at 1 mM
SDS
77% inhibition at 1 mM, 100% at 10 mM
SDS
WP_028494267
1 mM, 99% loss of activity
Tris
10 mM, 9% residual activity
Tris
58% inhibition at 5 mM, 78% at 10 mM
Tris
the enzyme-Tris complex may represent a substrate-induced closed conformation that facilitates intramolecular isomerization and minimize disaccharide hydrolysis
Tris
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complete inhibition at 25 mM, 23% inhibition of aggregated enzyme
Tris
structural characteristics of enzyme TtTS in complex with the inhibitor TriS
Tris
WP_028494267
1 mM, 46% loss of activity
Tris
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inhibition of recombinant fusion protein with N-terminal beta-amylase of Clostridium thermofluorogenes and C-terminal trehalose synthase or vice versa
validoxylamine
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strong inhibition
validoxylamine
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strong inhibition
Zn2+
1 mM, 67% loss of activity
Zn2+
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71.6% residual activity at 5 mM
Zn2+
1 mM, 29% residual activity
Zn2+
complete inhibition at 5 mM
Zn2+
59% residual activity at 1 mM
Zn2+
complete inhibition at 2 mM
Zn2+
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92% inhibition at 2 mM, 1% inhibition of aggregated enzyme
Zn2+
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10 mM, 20% residual activity
Zn2+
WP_028494267
1 mM, 33% loss of activity
Zn2+
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inhibition of recombinant fusion protein with N-terminal beta-amylase of Clostridium thermofluorogenes and C-terminal trehalose synthase or vice versa
additional information
TreS activity is not affected by EDTA
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additional information
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TreS activity is not affected by EDTA
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additional information
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not inhibited by acarbose
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additional information
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not inhibited by acarbose
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additional information
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no or poor inhibition by MgCl2, MnCl, BaCl2, CaCl2, SrCl2, NiCl2, CoCl2Al2(SO4), FeSO4, DTT, and EDTA
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