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5.4.99.16: maltose alpha-D-glucosyltransferase

This is an abbreviated version!
For detailed information about maltose alpha-D-glucosyltransferase, go to the full flat file.

Word Map on EC 5.4.99.16

Reaction

maltose
=
alpha,alpha-trehalose

Synonyms

57-KDa trehalose synthase (Saccharomyces cerevisiae), Dgeo_0537, DKY63_02445, DOT40_01605, DrTreS, DrTS, DR_2036, FM102_08285, HMPREF9086_3732, Maltose alpha-D-glucosylmutase, Maltose alpha-D-glucosyltransferase, Maltose glucosylmutase, MTase, MtTS, Protein (Saccharomyces cerevisiae clone pMB14 gene CIF reduced), Protein (Saccharomyces cerevisiae gene CIF1 reduced), PTO0069, PTTS, Synthase, trehalose, Synthase, trehalose (Pimelobacter strain R48 clone pBRM8 gene treS precursor reduced), Synthase, trehalose (Saccharomyces cerevisiae gene TPS1 subunit), Synthase, trehalose (Saccharomyces cerevisiae gene TSL1 subunit), Synthase, trehalose (Thermus aquaticus strain ATCC33923 clone pBTM5), TaTS, Tfu_0584, Trehalose synthase, Trehalose synthase (Pimelobacter strain R48 clone pBRM8 gene treS precursor reduced), Trehalose synthetase, TreS, treSB, TSase, Tter_0330, TtTreS, TtTS

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.16 maltose alpha-D-glucosyltransferase

pH Stability

pH Stability on EC 5.4.99.16 - maltose alpha-D-glucosyltransferase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
purified recombinant His6-tagged enzyme, 2 h, 30°C, inactivation
726558
4
purified recombinant His6-tagged enzyme, 2 h, 30°C, inactivation
726558
4 - 8
the enzyme can maintain very high activity (above 90%) at pH 4.0-8.0 for 5 h, below pH 5.0 the enzyme shows no activity
703606
5 - 10
recombinant immobilized enzyme, 70°C, 2 h, over 80% activity remaning within this range
746777
5 - 7
-
both enzyme and recombinant fusion protein with N-terminal beta-amylase of Clostridium thermofluorogenes and C-terminal trehalose synthase or vice versa, stable
680294
5 - 8.5
-
-
715078
5 - 9
recombinant free enzyme, 70°C, 2 h, over 80% activity remaning within this range
746777
5.5
purified recombinant His6-tagged enzyme, 2 h, 30°C, 50% activity remaining
726558
5.5 - 7.5
-
701844
5.5 - 8
-
wild-type
680304
5.5 - 9.5
-
60°C, 60 min, stable
3489
6 - 10
WP_028494267
stable for 6 h
759527
6 - 9
6.5 - 8
-
the enzyme activity declines significantly at pH above 6.5. At pH 8.0 a complete loss in enzyme activity is observed for both the free and the immobilized enzyme. At pH5.0, the immobilized enzyme exhibits a relative activity, 82.13%, higher than the free enzyme, 50.57%.
716805
6.6 - 7.4
-
30 min, more than 80% residual activity
681153
7 - 9
-
37°C, 1 h, stable
3493
7.6
purified recombinant His6-tagged enzyme, 2 h, 45°C and pH 7.6, stable
726558
8 - 10
-
recombinant cyclized TreS retains more than 85% of its activity at a pH range of 8.0 to 10.0, while the wild type kept only 80% of its activity under the same conditions. Wild-type and linear TreS lost about 20% and 15% of their original activities after incubation in this pH range, respectively
747324
9.5
purified recombinant His6-tagged enzyme, 2 h, 30°C, 50% activity remaining
726558