Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is a good candidate in the large-scale production of trehalose from maltose | uncultured bacterium |
Cloned (Comment) | Organism |
---|---|
gene treS, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic tree, recombinant overexression in Escherichia coli strain BL21(DE3), cloning in Escherichia coli strain D5alpha | uncultured bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | slight inhibition | uncultured bacterium | |
Hg2+ | - |
uncultured bacterium | |
additional information | no or poor inhibition by MgCl2, MnCl, BaCl2, CaCl2, SrCl2, NiCl2, CoCl2Al2(SO4), FeSO4, DTT, and EDTA | uncultured bacterium | |
Pb2+ | slight inhibition | uncultured bacterium | |
SDS | - |
uncultured bacterium | |
Tris | - |
uncultured bacterium | |
Zn2+ | - |
uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63300 | - |
x * 63300, about, sequence calculation | uncultured bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | uncultured bacterium | - |
alpha,alpha-trehalose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | - |
isolated from saline-alkali soil, gene treS | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | uncultured bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
133.5 | - |
purified recombinant enzyme, pH 9.0, 45°C | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | - |
uncultured bacterium | alpha,alpha-trehalose | - |
r | |
maltose | the recombinant enzyme has a 4.1fold higher catalytic efficientcy for maltose than for trehalose | uncultured bacterium | alpha,alpha-trehalose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 63300, about, sequence calculation | uncultured bacterium |
Synonyms | Comment | Organism |
---|---|---|
Trehalose synthase | - |
uncultured bacterium |
TreS | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
uncultured bacterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 55 | over 30% of maximal activity within this range | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | over 50% of maximal activity within this range | uncultured bacterium |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 13 | uncultured bacterium |