Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, 110% of initial activity | Deinococcus geothermalis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Deinococcus geothermalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 55% residual activity | Deinococcus geothermalis | |
Cu2+ | 1 mM, 5% residual activity | Deinococcus geothermalis | |
EDTA | inhibitory above 1 mM | Deinococcus geothermalis | |
Fe3+ | 1 mM, 60% residual activity | Deinococcus geothermalis | |
Hg2+ | 1 mM, 15% residual activity | Deinococcus geothermalis | |
Mg2+ | 1 mM, 75% residual activity | Deinococcus geothermalis | |
Tris | 10 mM, 9% residual activity | Deinococcus geothermalis | |
Zn2+ | 1 mM, 29% residual activity | Deinococcus geothermalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
254 | - |
maltose | pH 7.6, 40°C | Deinococcus geothermalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
6400 | - |
x * 63620, calculated, x * 6400, SDS-PAGE of recombinant protein | Deinococcus geothermalis |
63620 | - |
x * 63620, calculated, x * 6400, SDS-PAGE of recombinant protein | Deinococcus geothermalis |
132300 | - |
gel filtration, recombinant protein | Deinococcus geothermalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus geothermalis | Q1J0Z5 | locus Dgeo-0537 | - |
Deinococcus geothermalis 11300 | Q1J0Z5 | locus Dgeo-0537 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.95 | - |
pH 7.6, 40°C | Deinococcus geothermalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | - |
Deinococcus geothermalis | alpha,alpha-trehalose | - |
? | |
maltose | - |
Deinococcus geothermalis 11300 | alpha,alpha-trehalose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x * 63620, calculated, x * 6400, SDS-PAGE of recombinant protein | Deinococcus geothermalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Deinococcus geothermalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | more than 80% of maximum activity | Deinococcus geothermalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life 42 h | Deinococcus geothermalis |
55 | - |
8 h, 57% residual activity | Deinococcus geothermalis |
60 | - |
2 h, 20% residual activity | Deinococcus geothermalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31.9 | - |
maltose | pH 7.6, 40°C | Deinococcus geothermalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Deinococcus geothermalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.8 | more than 80% of maximum activity | Deinococcus geothermalis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Deinococcus geothermalis | calculated | - |
4.9 |