Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

5.4.99.16: maltose alpha-D-glucosyltransferase

This is an abbreviated version!
For detailed information about maltose alpha-D-glucosyltransferase, go to the full flat file.

Word Map on EC 5.4.99.16

Reaction

maltose
=
alpha,alpha-trehalose

Synonyms

57-KDa trehalose synthase (Saccharomyces cerevisiae), Dgeo_0537, DKY63_02445, DOT40_01605, DrTreS, DrTS, DR_2036, FM102_08285, HMPREF9086_3732, Maltose alpha-D-glucosylmutase, Maltose alpha-D-glucosyltransferase, Maltose glucosylmutase, MTase, MtTS, Protein (Saccharomyces cerevisiae clone pMB14 gene CIF reduced), Protein (Saccharomyces cerevisiae gene CIF1 reduced), PTO0069, PTTS, Synthase, trehalose, Synthase, trehalose (Pimelobacter strain R48 clone pBRM8 gene treS precursor reduced), Synthase, trehalose (Saccharomyces cerevisiae gene TPS1 subunit), Synthase, trehalose (Saccharomyces cerevisiae gene TSL1 subunit), Synthase, trehalose (Thermus aquaticus strain ATCC33923 clone pBTM5), TaTS, Tfu_0584, Trehalose synthase, Trehalose synthase (Pimelobacter strain R48 clone pBRM8 gene treS precursor reduced), Trehalose synthetase, TreS, treSB, TSase, Tter_0330, TtTreS, TtTS

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.16 maltose alpha-D-glucosyltransferase

Purification

Purification on EC 5.4.99.16 - maltose alpha-D-glucosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HiTrap Chelating HP column chromatography
-
native enzyme by ammonium sulfate fractionation, dialysis, and two steps of gel filtration, followed by ultrafiltration
-
Ni column chromatography
-
Ni column chromatography, DEAE-cellulose column chromatography, hydroxyapatite column chromatography, aminohexylagarose column chromatography, phenyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration
-
Ni-NTA column chromatography
Ni-NTAcolumn chromatography
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain (DE3) Rosetta pLysS by cobalt affinity chromatography and ultrafiltration
recombinant enzyme
recombinant enzyme from Echerichia coli strain BL21(DE3) to 98% purity
recombinant enzyme from Escherichia coli strain BL21(DE3)
-
recombinant fusion protein with N-terminal beta-amylase of Clostridium thermofluorogenes and C-terminal trehalose synthase and vice versa having both activities
-
recombinant His-tagged domainC-fusion mutant enzyme CgTreS-TtTreS-C from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged domainC-fusion mutant enzyme PpTreS-TtTreS-C from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged domainC-fusion mutant enzyme ScTreS-TtTreS-C from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged domainC-fusion mutant enzyme TmTreS-TtTreS-C from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type enzyme and domainC-fusion mutant enzymes from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and gel filtration
recombinant His6-tagged enzyme by nickel affinity chromatography
recombinant His6-tagged wild-type and chimeric mutant enzymes from Escherichia coli strain Rosetta-gami (DE3) by nickel affinity chromatography
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration