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Literature summary for 5.4.99.16 extracted from

  • Cho, Y.; Park, O.; Shin, H.
    Immobilization of thermostable trehalose synthase for the production of trehalose (2006), Enzyme Microb. Technol., 39, 108-113.
No PubMed abstract available

Application

Application Comment Organism
synthesis immobilization of recombinant enzyme on Eupergit C250L for the production of trehalose. Immobilization does not affect optimum pH value, optimum temperature is shifted from 45°C to 65°C. Immobilized enzyme is stable at 70°C for 16 days and can be used more than 10times in batch reaction. A maximum yield of 42% trehalose can be reached from 50 g/l maltose Thermus caldophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus caldophilus

Organism

Organism UniProt Comment Textmining
Thermus caldophilus
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Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermus caldophilus