Literature summary for 5.4.99.16 extracted from

Pan, Y.T.; Carroll, J.D.; Asano, N.; Pastuszak, I.; Edavana, V.K.; Elbein, A.D.
Trehalose synthase converts glycogen to trehalose (2008), FEBS J., 275, 3408-3420.

Search for more literature for 5.4.99.16

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Mycolicibacterium smegmatis
expressed in Escherichia coli	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	the MTase activity of TreS is inhibited by 5 mM Ca2+ and other divalent cations	Mycobacterium tuberculosis
Ca2+	the MTase activity of TreS is inhibited by 5 mM Ca2+ and other divalent cations	Mycolicibacterium smegmatis
additional information	not inhibited by acarbose	Mycobacterium tuberculosis
additional information	not inhibited by acarbose	Mycolicibacterium smegmatis
validoxylamine	strong inhibition	Mycobacterium tuberculosis
validoxylamine	strong inhibition	Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
10	-	maltose	in 40 mM potassium phosphate buffer (pH 6.8), at 37°C	Mycolicibacterium smegmatis
90	-	alpha,alpha-trehalose	in 40 mM potassium phosphate buffer (pH 6.8), at 37°C	Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65000	-	6 * 65000, SDS-PAGE	Mycolicibacterium smegmatis
65000	-	6 * 65000, SDS-PAGE	Mycobacterium tuberculosis
68000	-	6 * 68000, gel filtration	Mycolicibacterium smegmatis
68000	-	6 * 68000, gel filtration	Mycobacterium tuberculosis
390000	-	gel filtration	Mycolicibacterium smegmatis
390000	-	gel filtration	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-
Mycolicibacterium smegmatis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni column chromatography	Mycobacterium tuberculosis
Ni column chromatography, DEAE-cellulose column chromatography, hydroxyapatite column chromatography, aminohexylagarose column chromatography, phenyl-Sepharose column chromatography, and Sephacryl S-200 gel filtration	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
maltose	-	Mycolicibacterium smegmatis	alpha,alpha-trehalose	-	r
maltose	-	Mycobacterium tuberculosis	alpha,alpha-trehalose	-	r
additional information	TreS has also amylase activity by producing trehalose from glycogen or maltoheptaose	Mycolicibacterium smegmatis	?	-	?
additional information	TreS has also amylase activity by producing trehalose from glycogen or maltoheptaose	Mycobacterium tuberculosis	?	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 65000, SDS-PAGE	Mycolicibacterium smegmatis
homohexamer	6 * 65000, SDS-PAGE	Mycobacterium tuberculosis
homohexamer	6 * 68000, gel filtration	Mycolicibacterium smegmatis
homohexamer	6 * 68000, gel filtration	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
MTase	-	Mycolicibacterium smegmatis
MTase	-	Mycobacterium tuberculosis
Trehalose synthase	-	Mycolicibacterium smegmatis
Trehalose synthase	-	Mycobacterium tuberculosis
TreS	-	Mycolicibacterium smegmatis
TreS	-	Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000025	-	validoxylamine	in 40 mM potassium phosphate buffer (pH 6.8), at 37°C	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)