Literature summary for 5.4.99.16 extracted from

Chen, Y.; Xiao, C.; Chen, X.; Yang, L.; Qi, X.; Zheng, J.; Li, M.; Zhang, J.
Preparation of cross-linked enzyme aggregates in water-in-oil emulsion: application to trehalose synthase (2014), J. Mol. Catal. B, 100, 84-90.

No PubMed abstract available

Search for more literature for 5.4.99.16

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression in Escherichia coli	Meiothermus ruber

Protein Variants

Protein Variants	Comment	Organism
additional information	preparation of cross-linked enzyme aggregates in a water-in-oil emulsion, spherical aggregates of recombinant trehalose synthase are obtained through the emulsion based process, method development and evaluation	Meiothermus ruber

Inhibitors

Inhibitors	Comment	Organism	Structure
Al3+	complete inhibition at 2 mM, 8% inhibition of aggregated enzyme	Meiothermus ruber
Cu2+	complete inhibition at 2 mM, 23% inhibition of aggregated enzyme	Meiothermus ruber
Fe2+	95% inhibition at 2 mM, 5% inhibition of aggregated enzyme	Meiothermus ruber
Guanidine-HCl	complete inhibition at 1 M, 51% inhibition of aggregated enzyme	Meiothermus ruber
Tris	complete inhibition at 25 mM, 23% inhibition of aggregated enzyme	Meiothermus ruber
Urea	90% inhibition at 2 M, 1% inhibition of aggregated enzyme	Meiothermus ruber
Zn2+	92% inhibition at 2 mM, 1% inhibition of aggregated enzyme	Meiothermus ruber

Organism

Organism	UniProt	Comment	Textmining
Meiothermus ruber	-	-	-

Synonyms

Synonyms	Comment	Organism
Trehalose synthase	-	Meiothermus ruber
TreS	-	Meiothermus ruber

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
53	-	free enzyme	Meiothermus ruber
70	-	spherical aggregated enzyme	Meiothermus ruber

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	70	free enzyme	Meiothermus ruber
50	80	spherical aggregated enzyme	Meiothermus ruber

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	free enzyme	Meiothermus ruber
7	-	spherical aggregated enzyme	Meiothermus ruber

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	free and spherical aggregated enzyme	Meiothermus ruber

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Release 2023.1 (January 2023)