5.3.2.6: 2-hydroxymuconate tautomerase

This is an abbreviated version!
For detailed information about 2-hydroxymuconate tautomerase, go to the full flat file.

Word Map on EC 5.3.2.6

5.3.2.6

tautomerization

michael-type

trans-3-chloroacrylic

5-carboxymethyl-2-hydroxymuconate

2-oxo-3-pentynoate

stivers

dienolic

heterohexamer

2-hydroxy-2,4-pentadienoate

3-bromopyruvate

beta-alpha-beta

whitman

proline-based

3-chloroacrylic

cis-caad

meta-fission

cis-3-chloroacrylic

Reaction

Synonyms

4-OT, 4-oxalocrotonate tautomerase, 4-oxalocrotonate tautomerase I, 4-oxalocrotonate tautomerase II, 4OT, AfDmpI, D-4-oxalocrotonate tautomerase, HpDmpI, L-4-oxalocrotonate tautomerase, OCA, PRAC, SAR1376, TomN, xylH, YwhB

ECTree

5 Isomerases

5.3 Intramolecular oxidoreductases

5.3.2 Interconverting keto- and enol-groups

5.3.2.6 2-hydroxymuconate tautomerase

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Results	in table
1459	AA Sequence
1	CAS Registry Number
17	Cloned(Commentary)
7	Crystallization (Commentary)
34	General Information
27	Inhibitors
46	kcat/KM [mM/s]
1	Ki Value [mM]
50	KM Value [mM]
5	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
159	Organism
17	Pathway
146	PDB ID
13	pH Optimum
45	Protein Variants
13	Purification (Commentary)
6	Reaction
43	Reference
1	Source Tissue
124	Substrates and Products (Substrate)
30	Subunits
83	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
1	Temperature Stability [°C]
46	Turnover Number [1/s]

Purification

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Purification on EC 5.3.2.6 - 2-hydroxymuconate tautomerase

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4 entries

DEAE-Sepharose column chromatography and SP-Sepharose column chromatography

Methylibium petroleiphilum

A2SL37

726816

native enzyme from Escherichia coli strain C by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, ultrafiltration, ammoniums ulfate fractionation, and hydrophobic interaction chromatography, followed by gel filtration

Escherichia coli

719634

phenyl-Sepharose column chromatography and DEAE-Sepharose column chromatography

Pseudomonas putida

726964

recombinant enzyme from Escherichia coli by ultracentrifugation, two different steps of anion exchange chromatography, hydroxylapatite and hydrophobic interaction chromatography, and gel filtration with alternating steps of ultrafiltration

Pseudomonas putida

719633

recombinant hh4-OT wild-type and mutants from Escherichia coli by heat treatment, anion exchange chromatography, and gel-filtration

Chloroflexus aurantiacus

A9W9U6

718874

recombinant wild-type and mutant enzymes from Escherichia coli strain BL21-Gold(DE3) to over 95% purity

Pseudomonas putida

718844

recombinant wild-type and mutant YwhBs from Escherichia coli strain BL21(DE3)pLysS

Bacillus subtilis

718847

recombinant wild-type and mutants from Escherichia coli strain BL21(DE3)pLysS to homogeneity

Pseudomonas putida

718830