5.3.2.6: 2-hydroxymuconate tautomerase

This is an abbreviated version!
For detailed information about 2-hydroxymuconate tautomerase, go to the full flat file.

Reaction

Synonyms

4-OT, 4-oxalocrotonate tautomerase, 4-oxalocrotonate tautomerase I, 4-oxalocrotonate tautomerase II, 4OT, AfDmpI, D-4-oxalocrotonate tautomerase, HpDmpI, L-4-oxalocrotonate tautomerase, OCA, PRAC, SAR1376, TomN, xylH, YwhB

ECTree

     5 Isomerases

         5.3 Intramolecular oxidoreductases

             5.3.2 Interconverting keto- and enol-groups

                5.3.2.6 2-hydroxymuconate tautomerase

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Results	in table
1459	AA Sequence
1	CAS Registry Number
17	Cloned(Commentary)
7	Crystallization (Commentary)
34	General Information
27	Inhibitors
46	kcat/KM [mM/s]
1	Ki Value [mM]
50	KM Value [mM]
5	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
159	Organism
17	Pathway
146	PDB ID
13	pH Optimum
45	Protein Variants
13	Purification (Commentary)
6	Reaction
43	Reference
1	Source Tissue
124	Substrates and Products (Substrate)
30	Subunits
83	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
1	Temperature Stability [°C]
46	Turnover Number [1/s]

KM Value

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KM Value on EC 5.3.2.6 - 2-hydroxymuconate tautomerase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.334

(2E)-2-hydroxy-3-phenylprop-2-enoate

Pseudomonas putida

-

wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C

726964

0.05 - 0.17

(2Z,4E)-2-hydroxyhexa-2,4-dienedioate

5 entries

0.017 - 1.6

2-hydroxy-2,4-hexadienedioate

11 entries

0.09 - 1.11

2-hydroxy-2,4-pentadienoate

4 entries

0.017 - 1.05

2-hydroxymuconate

12 entries

0.189

2-oxo-3-hexenedioate

Pseudomonas putida

-

pH 7.3, 30°C

719633

0.09 - 0.103

2-oxo-4(E)-hexenedioate

2 entries

0.105 - 0.211

5-(methyl)-2-hydroxymuconate

2 entries

0.063 - 0.23

phenylenolpyruvate

8 entries

additional information

additional information

4 entries

-

0.05

(2Z,4E)-2-hydroxyhexa-2,4-dienedioate

Pseudomonas putida

-

at pH 7.3 and 22°C

747782

0.11

(2Z,4E)-2-hydroxyhexa-2,4-dienedioate

Helicobacter pylori

-

pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate

721792

0.11

(2Z,4E)-2-hydroxyhexa-2,4-dienedioate

Archaeoglobus fulgidus

O29588

pH 7.3, 23°C, conversion to compound 5

721792

0.135

(2Z,4E)-2-hydroxyhexa-2,4-dienedioate

Helicobacter pylori

-

pH 7.3, 23°C, conversion to compound 5

721792

0.17

(2Z,4E)-2-hydroxyhexa-2,4-dienedioate

Archaeoglobus fulgidus

O29588

pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate

721792

0.017

2-hydroxy-2,4-hexadienedioate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A

718874

0.069

2-hydroxy-2,4-hexadienedioate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A

718874

0.07

2-hydroxy-2,4-hexadienedioate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant wild-type hh4-OT

718874

0.135

2-hydroxy-2,4-hexadienedioate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A

718874

0.18

2-hydroxy-2,4-hexadienedioate

Pseudomonas putida

-

pH 7.3, 23°C, wild-type enzyme

718830

0.29

2-hydroxy-2,4-hexadienedioate

Pseudomonas putida

-

pH 7.3, 23°C, mutant R11A/R39A

718830

0.29

2-hydroxy-2,4-hexadienedioate

Pseudomonas putida

-

pH 7.3, 23°C, mutant R39A

718830

0.345

2-hydroxy-2,4-hexadienedioate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A

718874

0.47

2-hydroxy-2,4-hexadienedioate

Pseudomonas putida

-

pH 7.3, 23°C, mutant R39Q

718830

1.033

2-hydroxy-2,4-hexadienedioate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A

718874

1.6

2-hydroxy-2,4-hexadienedioate

Pseudomonas putida

-

pH 7.3, 23°C, mutant R11A

718830

0.09

2-hydroxy-2,4-pentadienoate

Archaeoglobus fulgidus

O29588

pH 7.3, 23°C

721792

0.19

2-hydroxy-2,4-pentadienoate

Helicobacter pylori

-

pH 7.3, 23°C

721792

1.11

2-hydroxy-2,4-pentadienoate

Pseudomonas putida

-

pH 7.3, 23°C, mutant R11A

718830

1.11

2-hydroxy-2,4-pentadienoate

Pseudomonas putida

-

pH 7.3, 23°C, wild-type enzyme

718830

0.017

2-hydroxymuconate

Pseudomonas putida

-

mutant 4-OT L8R, pH 7.3, 23°C

718844

0.027

2-hydroxymuconate

Pseudomonas putida

-

mutant 4-OT L8R/I52E, pH 7.3, 23°C

718844

0.06

2-hydroxymuconate

Methylibium petroleiphilum

A2SL37

isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C

726816

0.062

2-hydroxymuconate

Pseudomonas putida

-

mutant 4-OT I52E, pH 7.3, 23°C

718844

0.14

2-hydroxymuconate

Methylibium petroleiphilum

A2SL37

isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C

726816

0.145

2-hydroxymuconate

Pseudomonas putida

-

pH 7.3, 30°C

719633

0.18

2-hydroxymuconate

Pseudomonas putida

-

wild-type 4-OT, pH 7.3, 23°C

718844

0.18

2-hydroxymuconate

Pseudomonas putida

-

mutant enzyme P1A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C

726964

0.44

2-hydroxymuconate

Pseudomonas putida

-

mutant enzyme R39A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C

726964

0.512

2-hydroxymuconate

Pseudomonas putida

-

wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C

726964

0.78

2-hydroxymuconate

Pseudomonas putida

-

mutant enzyme R61A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C

726964

1.05

2-hydroxymuconate

Pseudomonas putida

-

mutant enzyme R11A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C

726964

0.09

2-oxo-4(E)-hexenedioate

Pseudomonas putida

-

D-4OT enantiomer, pH 7.3, 30°C

719636

0.103

2-oxo-4(E)-hexenedioate

Pseudomonas putida

-

L-4OT enantiomer, pH 7.3, 30°C

719636

0.105

5-(methyl)-2-hydroxymuconate

Methylibium petroleiphilum

A2SL37

isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C

726816

0.211

5-(methyl)-2-hydroxymuconate

Methylibium petroleiphilum

A2SL37

isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C

726816

0.063

phenylenolpyruvate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A

718874

0.09

phenylenolpyruvate

Archaeoglobus fulgidus

O29588

pH 7.3, 23°C

721792

0.121

phenylenolpyruvate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant wild-type hh4-OT

718874

0.143

phenylenolpyruvate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A

718874

0.152

phenylenolpyruvate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A

718874

0.159

phenylenolpyruvate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A

718874

0.198

phenylenolpyruvate

Chloroflexus aurantiacus

A9W9U6

pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A

718874

0.23

phenylenolpyruvate

Helicobacter pylori

-

pH 7.3, 23°C

721792

additional information

additional information

Pseudomonas putida

-

steady-state kinetics, overview

718830

-

additional information

additional information

Pseudomonas putida

-

4-OT exhibits hyperbolic kinetics and shows anticooperativity during catalysis

718840

-

additional information

additional information

Pseudomonas putida

-

kinetic modeling, overview

719633

-

additional information

additional information

Pseudomonas putida

-

steady-state kinetics of wild-type and mutant 4-OTs for trans-3-chloroacrylic acid dehalogenase activity, pH 8.2, 23°C, overview

718844

-