Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
x * 14000, 5-(carboxymethyl)-2-hydroxymuconate isomerase, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli C | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from Escherichia coli strain C by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, ultrafiltration, ammoniums ulfate fractionation, and hydrophobic interaction chromatography, followed by gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxymuconate | stereospecific ketonization | Pseudomonas putida | 2-oxo-3-(E)-hexenedioate | - |
? | |
2-hydroxymuconate | stereospecific ketonization, a reaction of EC 5.3.2.6, is also catalyzed by 5-(carboxymethyl)-2-hydroxymuconate isomerase, EC 5.3.3.10 | Escherichia coli | 2-oxo-3-(E)-hexenedioate | - |
? | |
2-hydroxymuconate | stereospecific ketonization | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | 2-oxo-3-(E)-hexenedioate | - |
? | |
2-hydroxymuconate | stereospecific ketonization, a reaction of EC 5.3.2.6, is also catalyzed by 5-(carboxymethyl)-2-hydroxymuconate isomerase, EC 5.3.3.10 | Escherichia coli C | 2-oxo-3-(E)-hexenedioate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 14000, 5-(carboxymethyl)-2-hydroxymuconate isomerase, SDS-PAGE | Escherichia coli |
? | x * 3600, 4-oxalocrotonate tautomerase, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
4-OT | - |
Pseudomonas putida |
4-oxalocrotonate tautomerase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Escherichia coli |
7.3 | - |
assay at | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
evolution | mechanism and the evolution of 4-oxalocrotonate tautomerase, and 5-(carboxymethyl)-2-hydroxymuconate isomerase, EC 5.3.3.10, and their respective pathways, overview | Pseudomonas putida |
evolution | mechanism and the evolution of 4-oxalocrotonate tautomerase, EC 5.3.2.6, and 5-(carboxymethyl)-2-hydroxymuconate isomerase and their respective pathways, overview | Escherichia coli |
metabolism | in the catechol meta-fission pathway elaborated by Pseudomonas putida mt-2 ketonization of 2-hydroxymuconate by 4-oxalocrotonate tautomerase generates the alpha,beta-unsaturated ketone 2-oxo-3-(E)-hexenedioate, which undergoes decarboxylation and further processing to intermediates in the Krebs cycle | Pseudomonas putida |
metabolism | in the homoprotocatechuate pathway elaborated by Escherichia coli C ketonization of 5-(carboxymethyl)-2-hydroxymuconate by 5-(carboxymethyl)-2-hydroxymuconate isomerase generates the alpha,beta-unsaturated ketone, which undergoes decarboxylation and further processing to intermediates in the Krebs cycle | Escherichia coli |