5.3.2.6: 2-hydroxymuconate tautomerase

This is an abbreviated version!
For detailed information about 2-hydroxymuconate tautomerase, go to the full flat file.

Reaction

Synonyms

4-OT, 4-oxalocrotonate tautomerase, 4-oxalocrotonate tautomerase I, 4-oxalocrotonate tautomerase II, 4OT, AfDmpI, D-4-oxalocrotonate tautomerase, HpDmpI, L-4-oxalocrotonate tautomerase, OCA, PRAC, SAR1376, TomN, xylH, YwhB

ECTree

     5 Isomerases

         5.3 Intramolecular oxidoreductases

             5.3.2 Interconverting keto- and enol-groups

                5.3.2.6 2-hydroxymuconate tautomerase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
1459	AA Sequence
1	CAS Registry Number
17	Cloned(Commentary)
7	Crystallization (Commentary)
34	General Information
27	Inhibitors
46	kcat/KM [mM/s]
1	Ki Value [mM]
50	KM Value [mM]
5	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
159	Organism
17	Pathway
146	PDB ID
13	pH Optimum
45	Protein Variants
13	Purification (Commentary)
6	Reaction
43	Reference
1	Source Tissue
124	Substrates and Products (Substrate)
30	Subunits
83	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
1	Temperature Stability [°C]
46	Turnover Number [1/s]

Crystallization

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Crystallization on EC 5.3.2.6 - 2-hydroxymuconate tautomerase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Crystallization (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

crystals are grown from 20% dioxane and 50% 2-methyl-2,4-pentanediol, pH 6.0, vapor diffusion method

Archaeoglobus fulgidus

O29588

721792

purified recombinant hh4-OT, by sitting drop vapour diffusion method, 0.001 ml of 20 mg/mL protein in 10 mM Na/KPO4 buffer, pH 7.3, is mixed in a 1:1 ratio with precipitant against a 0.1 ml reservoir solution, and by hanging drop vapor diffusion method with a 0.5 ml reservoir and 0.004 ml hanging drops that contain protein and precipitant, 0.25 M (NH4)2SO4 and 4% PEG 4000, in a 1:1 ratio, 3-5 days, X-ray diffraction structure determination and analysis at 2.41 A resolution

Chloroflexus aurantiacus

A9W9U6

718874

crystals are grown from 28% polyethylene glycol 400, 200 mM CaCl2, 0.1 M HEPES buffer (pH 7.5), vapor diffusion method

Helicobacter pylori

-

721792

hanging drop vapor diffusion method, using 200 mM tri-ammonium citrate and 10% (w/v)polyethylene glycol (PEG) 3350

Methylibium petroleiphilum

A2SL37

726816

mutant enzyme M45Y/F50A, sitting drop vapor diffusion method, using 0.2 M sodium formate, 0.1 M bis-tris propane pH 8.5 and 20% PEG 3350 (w/v)

Pseudomonas putida

Q01468

748742

sitting drop vapor diffusion method, using 100 mM magnesium acetate/100 mM sodium acetate, 5%-21% (w/v) PEG 8000, pH 4.5, and 100 mM calcium acetate/100 mM sodium acetate, 1%-13% (w/v) PEG 4000, pH 4.5

Pseudomonas putida

-

726964

4-OT from 80% saturated ammonium sulfate solution containing 100 mM HEPES, pH 7.5, and 1% PEG 200, 20°C, X-ray diffraction structure determination and analysis at 1.9-2.8 A resolution, heavy etal derivatization by soaking the crystals for 4 h in crystallization solution containing 2 mM K2PtCl4

Pseudomonas sp.

P49172

718825