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0.031 - 170
D-fructose 6-phosphate
1.029
D-galactose
-
pH 8.0, 40°C
0.084 - 267.4
D-glucose 6-phosphate
0.25 - 1.1
D-mannose 6-phosphate
0.01 - 0.74
fructose 6-phosphate
0.03 - 8
glucose 6-phosphate
133
L-talose
pH 7.0, 95°C
additional information
additional information
-
0.031
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant S278L
0.034
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant I525T
0.037
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant wild-type enzyme
0.038
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant R347H
0.038
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant R75G
0.039
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant L487F
0.04
D-fructose 6-phosphate
-
50°C, pH 6.3
0.045
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant A300P
0.045
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant L339P
0.046
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant R347C
0.046
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant T375R
0.05
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant E495K
0.06
D-fructose 6-phosphate
-
pH 7.4, 80°C
0.061
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant R83W
0.063
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant T195I
0.063
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant V101M
0.068
D-fructose 6-phosphate
pH 7.5, 30°C, recombinant mutant R472H
0.14
D-fructose 6-phosphate
Cassia coluteoides
-
isoenzyme PGI I from germinating seeds
0.1425
D-fructose 6-phosphate
-
-
0.147
D-fructose 6-phosphate
-
22°C, pH 7.4
0.18
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme Y95K
0.19
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme H136A
0.2
D-fructose 6-phosphate
80°C, pH 7.4
0.21
D-fructose 6-phosphate
50°C, pH 7.4
0.22
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme T63A
0.23
D-fructose 6-phosphate
Cassia coluteoides
-
isoenzyme PGI II from developing seeds
0.25
D-fructose 6-phosphate
pH 7.4, 70°C, wild-type enzyme
0.27
D-fructose 6-phosphate
-
pH 7.6, 25°C
0.3
D-fructose 6-phosphate
-
pH 7.4, 50°C
0.318
D-fructose 6-phosphate
-
pH 7.6, 22°C
0.35
D-fructose 6-phosphate
Cassia coluteoides
-
isoenzyme PGI II from germinating seeds
0.36
D-fructose 6-phosphate
-
pH 8.0
0.36
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme Y95F
0.39
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme H80D
0.42
D-fructose 6-phosphate
50°C, pH 7.0, recombinant enzyme
0.44
D-fructose 6-phosphate
80°C, pH 7.4
0.46
D-fructose 6-phosphate
Cassia coluteoides
-
isoenzyme PGI I from developing seeds
0.5
D-fructose 6-phosphate
50°C
0.5
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme G79A
0.59
D-fructose 6-phosphate
50°C, pH 7.5, mutant enzyme E89V
0.6
D-fructose 6-phosphate
-
pH 7.4, 50°C
0.6 - 3
D-fructose 6-phosphate
pH 7.0, 50°C
0.63
D-fructose 6-phosphate
50°C, pH 7.0, native enzyme
0.7
D-fructose 6-phosphate
-
pH 7.6, 37°C
0.8
D-fructose 6-phosphate
-
pH 7.4, 50°C
1
D-fructose 6-phosphate
-
80°C, pH 7.0, native enzyme
1.2
D-fructose 6-phosphate
-
80°C, pH 7.0, recombinant enzyme
1.4
D-fructose 6-phosphate
50°C, pH 7.5, mutant enzyme H137A
1.4
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme Y160F
1.7
D-fructose 6-phosphate
50°C, pH 7.5, wild-type enzyme
2
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme G79L
2
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme H82A
2.1
D-fructose 6-phosphate
50°C, pH 7.5, mutant enzyme H91A
2.2
D-fructose 6-phosphate
-
at pH 6.0 and 37°C
2.9
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme E93D
3
D-fructose 6-phosphate
-
pH 7.2, 37°C
3.5
D-fructose 6-phosphate
50°C, pH 7.5, mutant enzyme H89A
4
D-fructose 6-phosphate
-
80°C, pH not specified in the publication
20.3
D-fructose 6-phosphate
pH 7.4, 70°C, mutant enzyme H80A
169
D-fructose 6-phosphate
-
wild-type, 37°C, pH 8.0
170
D-fructose 6-phosphate
-
mutant A346H, 37°C, pH 8.0
0.084
D-glucose 6-phosphate
-
in 50 mM HEPES buffer, pH 7.1, at 25°C
0.11
D-glucose 6-phosphate
-
ZR-82 cells, 22°C
0.18
D-glucose 6-phosphate
mutant enzyme D511N, at 21°C in 20 mM HEPES buffer (pH 7.5)
0.2 - 1
D-glucose 6-phosphate
mutant enzyme H100L, at 21°C in 20 mM HEPES buffer (pH 7.5)
0.23
D-glucose 6-phosphate
mutant enzyme E495Q, at 21°C in 20 mM HEPES buffer (pH 7.5)
0.28
D-glucose 6-phosphate
-
22°C, pH 7.4
0.29
D-glucose 6-phosphate
mutant enzyme H396L, at 21°C in 20 mM HEPES buffer (pH 7.5)
0.29
D-glucose 6-phosphate
wild type enzyme, at 21°C in 20 mM HEPES buffer (pH 7.5)
0.3
D-glucose 6-phosphate
mutant enzyme Y274F, at 21°C in 20 mM HEPES buffer (pH 7.5)
0.3
D-glucose 6-phosphate
mutant enzyme Y341F, at 21°C in 20 mM HEPES buffer (pH 7.5)
0.4
D-glucose 6-phosphate
-
pH 7.4, 50°C
0.5
D-glucose 6-phosphate
mutant enzyme Q388A, at 21°C in 20 mM HEPES buffer (pH 7.5)
0.6
D-glucose 6-phosphate
-
pH 7.4, 50°C
0.72
D-glucose 6-phosphate
80°C, pH 7.4
0.73
D-glucose 6-phosphate
mutant enzyme S185A, at 21°C in 20 mM HEPES buffer (pH 7.5)
0.78
D-glucose 6-phosphate
mutant enzyme N386A, at 21°C in 20 mM HEPES buffer (pH 7.5)
0.83
D-glucose 6-phosphate
Cassia coluteoides
-
isoenzyme PGI II from germinating seeds
1
D-glucose 6-phosphate
-
pH 7.6, 37°C
1
D-glucose 6-phosphate
-
pH 7.2, 37°C
1
D-glucose 6-phosphate
-
50°C, pH 6.3
1.02
D-glucose 6-phosphate
-
cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C
1.04
D-glucose 6-phosphate
mutant enzyme N154Q, at 21°C in 20 mM HEPES buffer (pH 7.5)
1.1
D-glucose 6-phosphate
Cassia coluteoides
-
isoenzyme PGI I from germinating seeds
1.3
D-glucose 6-phosphate
Cassia coluteoides
-
isoenzyme PGI II from developing seeds
1.5
D-glucose 6-phosphate
Cassia coluteoides
-
isoenzyme PGI I from developing seeds
1.53
D-glucose 6-phosphate
-
avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C
1.65
D-glucose 6-phosphate
-
avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C
1.86
D-glucose 6-phosphate
-
cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C
1.9
D-glucose 6-phosphate
-
pH 7.4, 80°C
1.9
D-glucose 6-phosphate
-
free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C
1.99
D-glucose 6-phosphate
pH 7.0, 50°C
1.99
D-glucose 6-phosphate
50°C, pH 7.0, native enzyme
2
D-glucose 6-phosphate
50°C, pH 7.0, recombinant enzyme
2.11
D-glucose 6-phosphate
-
immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C
2.43
D-glucose 6-phosphate
-
immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C
2.58
D-glucose 6-phosphate
-
mutant GroD1 cells, 22°C
2.7
D-glucose 6-phosphate
-
pH 7.4, 50°C
2.89
D-glucose 6-phosphate
-
free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C
3.5
D-glucose 6-phosphate
80°C, pH 7.4
7.9
D-glucose 6-phosphate
-
80°C, pH 7.0, recombinant enzyme
8.7
D-glucose 6-phosphate
-
80°C, pH 7.0, native enzyme
11.7
D-glucose 6-phosphate
50°C, pH 7.5, wild-type enzyme
221
D-glucose 6-phosphate
-
wild-type, 37°C, pH 8.0
267.4
D-glucose 6-phosphate
-
mutant A346H, 37°C, pH 8.0
0.25
D-mannose 6-phosphate
80°C, pH 7.4
1.1
D-mannose 6-phosphate
80°C, pH 7.4
0.01 - 0.17
fructose 6-phosphate
-
muscle enzyme, values of 0.01 mM, 0.12 mM and 0.17 mM are determined by different authors
0.0186
fructose 6-phosphate
-
isomerase a
0.0205
fructose 6-phosphate
-
isomerase c
0.0213
fructose 6-phosphate
-
isomerase b
0.048
fructose 6-phosphate
-
-
0.0596
fructose 6-phosphate
-
mutant enzyme Thr224 to Met
0.0635
fructose 6-phosphate
-
native enzyme
0.0657
fructose 6-phosphate
-
mutant enzyme Thr5 to Ile
0.07
fructose 6-phosphate
-
mammary gland enzyme
0.071
fructose 6-phosphate
-
-
0.0734
fructose 6-phosphate
-
mutant enzyme Asp539 to Asn
0.0769
fructose 6-phosphate
-
mutant enzyme Gln343 to Arg
0.09
fructose 6-phosphate
-
isozyme 1 and isozyme 2
0.1
fructose 6-phosphate
-
-
0.1
fructose 6-phosphate
-
-
0.1
fructose 6-phosphate
-
-
0.1
fructose 6-phosphate
-
-
0.1
fructose 6-phosphate
-
fructose 6-phosphate, isozyme 4
0.1
fructose 6-phosphate
-
glucose 6-phosphate, soluble enzyme
0.11
fructose 6-phosphate
-
isozyme 3
0.11 - 0.23
fructose 6-phosphate
-
values of 0.11 mM, 0.15 mM and 0.23 mM are determined by different autors
0.116
fructose 6-phosphate
-
-
0.119
fructose 6-phosphate
-
-
0.12
fructose 6-phosphate
-
-
0.12
fructose 6-phosphate
-
liver enzyme
0.12
fructose 6-phosphate
-
fructose 6-phosphate, enzyme form PGI II
0.12
fructose 6-phosphate
-
fructose 6-phosphate, at pH 8.6
0.12
fructose 6-phosphate
-
glucose 6-phosphate
0.122
fructose 6-phosphate
-
-
0.167
fructose 6-phosphate
-
-
0.17
fructose 6-phosphate
-
enzyme form PGI I
0.18
fructose 6-phosphate
-
-
0.2
fructose 6-phosphate
-
glucose 6-phosphate, immobilized enzyme
0.2
fructose 6-phosphate
-
enzyme forms PGI I and PGI II
0.21
fructose 6-phosphate
-
erythrocyte enzyme, wild-type
0.228 - 0.278
fructose 6-phosphate
-
-
0.3
fructose 6-phosphate
-
isozyme 1 and 2
0.46
fructose 6-phosphate
-
glucose 6-phosphate, isozyme 2
0.48
fructose 6-phosphate
-
chloroplastic isoenzyme
0.74
fructose 6-phosphate
-
erythrocyte enzyme, mutant B9
0.03 - 0.8
glucose 6-phosphate
-
muscle enzyme, values of 0.03 mM, 0.31 mM and 0.8 mM are determined by different authors
0.12 - 0.57
glucose 6-phosphate
-
mammary gland enzyme, values of 0.12 mM and 0.57 mM are determined by different authors
0.25
glucose 6-phosphate
-
-
0.27
glucose 6-phosphate
-
-
0.3 - 1.5
glucose 6-phosphate
-
values of 0.27 mM, 0.3 mM, 0.7 mM, 0.8 mM and 1.5 mM are determined by different authors
0.351
glucose 6-phosphate
-
mutant enzyme Thr224 to Met
0.36
glucose 6-phosphate
-
-
0.44
glucose 6-phosphate
-
glucose 6-phosphate
0.44
glucose 6-phosphate
-
glucose 6-phosphate, enzyme form PGI I and PGI II
0.44
glucose 6-phosphate
-
at pH 8.6
0.445
glucose 6-phosphate
-
native enzyme
0.449
glucose 6-phosphate
-
mutant enzyme Thr5 to Ile
0.45
glucose 6-phosphate
-
isozyme 4
0.505
glucose 6-phosphate
-
mutant enzyme Asp539 to Asn
0.51
glucose 6-phosphate
-
isozyme 1
0.573
glucose 6-phosphate
-
mutant enzyme Gln343 to Arg
0.58
glucose 6-phosphate
-
isozyme 3
0.58
glucose 6-phosphate
-
fructose 6-phosphate, cytosolic isoenzame
0.6
glucose 6-phosphate
-
liver enzyme
2
glucose 6-phosphate
-
-
2
glucose 6-phosphate
-
-
5.9
glucose 6-phosphate
-
isozyme 2
8
glucose 6-phosphate
-
isozyme 1
8
glucose 6-phosphate
-
glucose 6-phosphate, cytosolic and chloroplastic isoenzyme
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
the pH-value has a great influence on the Km-value for fructose 6-phosphate
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
kinetics for aldose substrates, overview
-
additional information
additional information
-
kinetics for aldose substrates, overview
-