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Literature summary for 5.3.1.9 extracted from

  • Gracy, R.W.; Tilley, B.E.
    Phosphoglucose isomerase of human erythrocytes and cardiac tissue (1975), Methods Enzymol., 41B, 392-400.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.071
-
fructose 6-phosphate
-
Homo sapiens
0.12
-
fructose 6-phosphate glucose 6-phosphate Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
2 * 63000, sedimentation velocity ultracentrifugation of enzyme dissociated in 6 M guanidine/HCl Homo sapiens
132000
-
sedimentation velocity ultracentrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Oxidation Stability

Oxidation Stability Organism
susceptible to oxidation of sulfhydryl groups yielding multiple electrophoretic forms with catalytic activity Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-
heart
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
best storage conditions: as crystalline suspension or precipitate in 0.70 saturated ammonium sulfate containing 50 mM triethanolamine buffer, pH 8.3, 1 mM EDTA, 1 mM dithiothreitol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fructose 6-phosphate r Homo sapiens Glucose 6-phosphate
-
?
Glucose 6-phosphate r Homo sapiens Fructose 6-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 63000, sedimentation velocity ultracentrifugation of enzyme dissociated in 6 M guanidine/HCl Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 10 about 50% of maximal activity at pH 7 and at pH 10 Homo sapiens