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Literature summary for 5.3.1.9 extracted from

  • Verhees, C.H.; Huynen, M.A.; Ward, D.E.; Schiltz, E.; De Vos, W.M.; van der Oost, J.
    The phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus is a unique glycolytic enzyme that belongs to the cupin superfamily (2001), J. Biol. Chem., 276, 40926-40932.
    View publication on PubMed
Search for more literature for 5.3.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Pyrococcus furiosus
functionally expressed in Escherichia coli BL21(DE3) Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
D-fructose 1,6-bisphosphate 10 mM, residual activities are 41% and 53% in the direction of fructose 6-phosphate and glucose 6-phosphate formation, respectively Pyrococcus furiosus
D-Fructose 1-phosphate 2 mM, residual activities are 50% and 69% in the direction of fructose 6-phosphate and glucose 6-phosphate formation, respectively Pyrococcus furiosus
D-mannose 6-phosphate 1.25 mM, residual activities are 18% and 38% in the direction of fructose 6-phosphate and glucose 6-phosphate formation, respectively Pyrococcus furiosus
additional information activity is not affected by addition of 10 mM EDTA. The addition of fructose, glucose, mannose, galactose (10 mM), pyruvate, phosphoenolpyruvate (10 mM), AMP, ADP, or ATP (3.5 mM), does not show any effect on the activity neither in the fructose 6-phosphate formation, nor in the glucose 6-phosphate formation Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
 D-fructose 6-phosphate 50°C, pH 7.0, recombinant enzyme Pyrococcus furiosus
0.6 3 D-fructose 6-phosphate pH 7.0, 50°C Pyrococcus furiosus
0.63
-
 D-fructose 6-phosphate 50°C, pH 7.0, native enzyme Pyrococcus furiosus
1.99
-
 D-glucose 6-phosphate pH 7.0, 50°C Pyrococcus furiosus
1.99
-
 D-glucose 6-phosphate 50°C, pH 7.0, native enzyme Pyrococcus furiosus
2
-
 D-glucose 6-phosphate 50°C, pH 7.0, recombinant enzyme Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity is not affected by addition of Mg2+ or Mn2+ Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21476
-
 2 * 21476, calculated from sequence Pyrococcus furiosus
23000
-
 2 * 23000, SDS-PAGE Pyrococcus furiosus
23500
-
 2 * 23500, SDS-PAGE Pyrococcus furiosus
49600
-
 gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus P83194
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.5
-
 50°C, pH 7.0, reaction with D-fructose 6-phosphate Pyrococcus furiosus
14.5
-
 pH 7.0, 50°C, substrate: D-fructose 6-phosphate Pyrococcus furiosus
29.1
-
 50°C, pH 7.0, reaction with D-glucose 6-phosphate Pyrococcus furiosus
29.1
-
 pH 7.0, 50°C, substrate: D-glucose 6-phosphate Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate
-
 Pyrococcus furiosus D-glucose 6-phosphate
-
 r
D-glucose 6-phosphate
-
 Pyrococcus furiosus D-fructose 6-phosphate
-
 r
additional information enzyme does not convert mannose 6-phosphate to fructose 6-phosphate Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23000, SDS-PAGE Pyrococcus furiosus
homodimer 2 * 23500, SDS-PAGE Pyrococcus furiosus
homodimer 2 * 21476, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
pgiA
-
 Pyrococcus furiosus
Phosphoglucose isomerase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Pyrococcus furiosus
90
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 115 70°C: about 40% of maximal activity, 115°C: about 50% of maximal activity Pyrococcus furiosus
70 110 70°C: about 40% of maximal activity, 110°C: about 35% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 half-life: 2.4 h Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 both directions Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 the enzyme shows reversible isomerization activity with fructose 6-phosphate and glucose 6-phosphate between pH 6.0 and pH 8.5 Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function the enzyme is part of the Pyrococcus furiosus variant of the Embden-Meyerhof pathway Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2
-
 D-glucose 6-phosphate pH 7.0, 50°C, native enzyme Pyrococcus furiosus
8.5
-
 D-glucose 6-phosphate pH 7.0, 50°C, recombinant enzyme Pyrococcus furiosus
11.5
-
 D-fructose 6-phosphate pH 7.0, 50°C, native enzyme Pyrococcus furiosus
16.5
-
 D-fructose 6-phosphate pH 7.0, 50°C, recombinant enzyme Pyrococcus furiosus