Literature summary for 5.3.1.9 extracted from

Jeong, J.J.; Fushinobu, S.; Ito, S.; Jeon, B.S.; Shoun, H.; Wakagi, T.
Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis (2003), FEBS Lett., 535, 200-204.

Search for more literature for 5.3.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Thermococcus litoralis

Protein Variants

Protein Variants	Comment	Organism
E98V	the ratio of turnover number and Km-value is 31373fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal	Thermococcus litoralis
H137A	the ratio of turnover number and Km-value is 66.7fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal	Thermococcus litoralis
H89A	the ratio of turnover number and Km-value is 2712fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal	Thermococcus litoralis
H91A	the ratio of turnover number and Km-value is 66.7fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal	Thermococcus litoralis

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	10 mM, 96% inhibition, D-fructose 6-phosphate as substrate	Thermococcus litoralis
Co2+	10 mM, 59% inhibition, D-fructose 6-phosphate as substrate	Thermococcus litoralis
Cu2+	10 mM, 96% inhibition, D-fructose 6-phosphate as substrate	Thermococcus litoralis
D-fructose 1,6-bisphosphate	-	Thermococcus litoralis
D-Fructose 1-phosphate	-	Thermococcus litoralis
D-gluconate 6-phosphate	-	Thermococcus litoralis
D-mannose 6-phosphate	-	Thermococcus litoralis
EDTA	10 mM, 78% inhibition, D-fructose 6-phosphate as substrate	Thermococcus litoralis
K+	10 mM, 18% inhibition, D-fructose 6-phosphate as substrate	Thermococcus litoralis
Mn2+	10 mM, 18% inhibition, D-fructose 6-phosphate as substrate	Thermococcus litoralis
Ni2+	10 mM, 84% inhibition, D-fructose 6-phosphate as substrate	Thermococcus litoralis
Zn2+	10 mM, 89% inhibition, D-fructose 6-phosphate as substrate	Thermococcus litoralis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.59	-	D-fructose 6-phosphate	50°C, pH 7.5, mutant enzyme E89V	Thermococcus litoralis
1.4	-	D-fructose 6-phosphate	50°C, pH 7.5, mutant enzyme H137A	Thermococcus litoralis
1.7	-	D-fructose 6-phosphate	50°C, pH 7.5, wild-type enzyme	Thermococcus litoralis
2.1	-	D-fructose 6-phosphate	50°C, pH 7.5, mutant enzyme H91A	Thermococcus litoralis
3.5	-	D-fructose 6-phosphate	50°C, pH 7.5, mutant enzyme H89A	Thermococcus litoralis
11.7	-	D-glucose 6-phosphate	50°C, pH 7.5, wild-type enzyme	Thermococcus litoralis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	10 mM, 37°C, 156% enhancement of the activity of the wild-type enzyme, mutant enzymes H89A, H91A, E98V and H137A do not contain iron or zinc	Thermococcus litoralis
Iron	wild-type enzyme contains 1.25 mol iron per mol of dimer, mutant enzymes H89A, H91A, E98V and H137A do not contain iron or zinc	Thermococcus litoralis
Zinc	wild-type enzyme contains 0.24 mol zinc per mol of dimer	Thermococcus litoralis

Organism

Organism	UniProt	Comment	Textmining
Thermococcus litoralis	P84140	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermococcus litoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
47.3	-	formation of D-glucose 6-phosphate	Thermococcus litoralis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
fructose 6-phosphate	-	Thermococcus litoralis	D-glucose 6-phosphate	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0003	-	D-fructose 6-phosphate	50°C, pH 7.5, mutant enzyme E98V	Thermococcus litoralis
0.021	-	D-fructose 6-phosphate	50°C, pH 7.5, mutant enzyme H89A	Thermococcus litoralis
0.34	-	D-fructose 6-phosphate	50°C, pH 7.5, mutant enzyme H137A	Thermococcus litoralis
0.5	-	D-fructose 6-phosphate	50°C, pH 7.5, mutant enzyme H91A	Thermococcus litoralis
26	-	D-fructose 6-phosphate	50°C, pH 7.5, wild-type enzyme	Thermococcus litoralis
42	-	D-glucose 6-phosphate	50°C, pH 7.5, wild-type enzyme	Thermococcus litoralis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.38	-	D-gluconate 6-phosphate	50°C, pH 7.5, D-fructose 6-phosphate as substrate	Thermococcus litoralis
2.3	-	D-mannose 6-phosphate	50°C, pH 7.5, D-fructose 6-phosphate as substrate	Thermococcus litoralis
4	-	D-Fructose 1-phosphate	50°C, pH 7.5, D-fructose 6-phosphate as substrate	Thermococcus litoralis
12	-	D-fructose 1,6-bisphosphate	50°C, pH 7.5, D-fructose 6-phosphate as substrate	Thermococcus litoralis

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Release 2023.1 (January 2023)