5.3.1.5: xylose isomerase
This is an abbreviated version!
For detailed information about xylose isomerase, go to the full flat file.
Word Map on EC 5.3.1.5
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5.3.1.5
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isomerization
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biomass
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d-glucose
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xylitol
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xylulokinase
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pentose
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lignocellulosic
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isomerases
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syrup
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corn
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gi
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d-fructose
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rubiginosus
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bioethanol
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piromyces
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arthrobacter
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l-arabinose
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synthesis
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actinoplanes
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high-fructose
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xylose-fermenting
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transaldolase
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saccharification
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thermoanaerobacter
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co-fermentation
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hemicellulosic
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thermoanaerobacterium
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neapolitana
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stipitis
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energy production
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orpinomyces
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thermosulfurogenes
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xylose-utilizing
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diauxic
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food industry
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nutrition
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biotechnology
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degradation
- 5.3.1.5
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isomerization
- biomass
- d-glucose
- xylitol
- xylulokinase
- pentose
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lignocellulosic
- isomerases
- syrup
- corn
- gi
- d-fructose
- rubiginosus
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bioethanol
- piromyces
- arthrobacter
- l-arabinose
- synthesis
- actinoplanes
-
high-fructose
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xylose-fermenting
- transaldolase
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saccharification
- thermoanaerobacter
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co-fermentation
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hemicellulosic
- thermoanaerobacterium
- neapolitana
- stipitis
- energy production
- orpinomyces
- thermosulfurogenes
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xylose-utilizing
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diauxic
- food industry
- nutrition
- biotechnology
- degradation
Reaction
Synonyms
D-XI, D-xylose aldose-ketose-isomerase, D-xylose isomerase, D-Xylose ketoisomerase, D-xylose ketol isomerase, D-xylose ketol-isomerase, D-xylose: ketol-isomerase, D-xylulose keto-isomerase, glucose isomerase, glucose/xylose isomerase, GXI, Isomerase, xylose, Maxazyme, Optisweet, SDXyI, Spezyme, Sweetase, Sweetzyme, Sweetzyme Q, Swetase, T80 xylose isomerase, TcaXI, TNXI, TthXI, XI, XYLA, XylC, xylose (glucose) isomerase, xylose isomerase
ECTree
5.3.1.5 xylose isomeraseAdvanced search results
results (
results (KM Value
KM Value on EC 5.3.1.5 - xylose isomerase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
3
D-glyceraldehyde
in D2O, 24 mM imidazole buffer, 10 mM MgCl2, at pH 7.5 and 25°C
180
D-glucose
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60°C, pH 7.3, with Mg2+ and Co2+, mutant D81A and wild-type
200
D-glucose
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60°C, pH 7.3, with Mg2+ and Co2+, mutant D163N/E167Q
259.6
D-glucose
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enzyme isoform T90, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C
259.6
D-glucose
Opuntia vulgaris
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enzyme isoform T90, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C
310
D-glucose
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60°C, pH 7.3, with Mg2+ and Co2+, mutants E221A and D56N
1 - 4
D-xylose
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recombinant His12-tagged wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C
1 - 1.2
D-xylose
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mutant enzyme E186Q/N215D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
3
D-xylose
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native wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
3.4
D-xylose
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mutant enzyme N215D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
3.8
D-xylose
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recombinant His12-tagged wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
4 - 5
D-xylose
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mutant enzyme D57N, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C
4.3
D-xylose
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mutant enzyme K289H, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
4.9
D-xylose
in D2O, 24 mM imidazole buffer, 10 mM MgCl2, at pH 7.5 and 25°C
5
D-xylose
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recombinant His6-tagged wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
5.3
D-xylose
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mutant enzyme D57N, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
6
D-xylose
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mutant enzyme N247D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
6.5
D-xylose
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mutant enzyme Q256D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
7.4
D-xylose
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mutant enzyme D57H, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
8
D-xylose
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mutant enzyme K289E, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C
10
D-xylose
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mutant enzyme N215D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C
15
D-xylose
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mutant enzyme E186Q/N215D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C
27
D-xylose
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mutant enzyme K289H, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C
33
D-xylose
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recombinant His6-tagged wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C
37
D-xylose
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mutant enzyme K289E, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C
42
D-xylose
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mutant enzyme N247D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C
46.4
D-xylose
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enzyme isoform T90, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C
46.4
D-xylose
Opuntia vulgaris
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enzyme isoform T90, in 50 mM Tris-HCl, pH 7.5, containing 1 mM CoCl2, at 90°C
50.18
D-xylose
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mutant enzyme E129D/V433I/E15D/E114G, in 100 mM Tris-HCl buffer at pH 7.5 and 30°C
54.03
D-xylose
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in 100 mM Tris-HCl buffer (pH 7.5), 10 mM MgCl2, at 30°C
83
D-xylose
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native wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C
86.97
D-xylose
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wild type enzyme, in 100 mM Tris-HCl buffer at pH 7.5 and 30°C
89.98
D-xylose
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mutant enzyme E129D/V433I, in 100 mM Tris-HCl buffer at pH 7.5 and 30°C
168.4
D-xylose
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mutant enzyme E129D/V433I/E15D/E114G/T142S/A177T, in 100 mM Tris-HCl buffer at pH 7.5 and 30°C
548
D-xylose
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mutant enzyme D57H, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C
additional information
additional information
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Km-values for wild-type enzyme and mutants E186D and E186Q, activated by Mg2+, Mn2+ or Co2+
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additional information
additional information
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Km-value for D-xylose varies with the concentration Mn2+
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