Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces rubiginosus |
Protein Variants | Comment | Organism |
---|---|---|
D287N | inactive | Streptomyces rubiginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | 4 | D-xylose | recombinant His12-tagged wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
1 | 1.2 | D-xylose | mutant enzyme E186Q/N215D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
3 | - |
D-xylose | native wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
3.4 | - |
D-xylose | mutant enzyme N215D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
3.8 | - |
D-xylose | recombinant His12-tagged wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
4 | 5 | D-xylose | mutant enzyme D57N, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
4.3 | - |
D-xylose | mutant enzyme K289H, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
5 | - |
D-xylose | recombinant His6-tagged wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
5.3 | - |
D-xylose | mutant enzyme D57N, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
6 | - |
D-xylose | mutant enzyme N247D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
6.5 | - |
D-xylose | mutant enzyme Q256D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
7.4 | - |
D-xylose | mutant enzyme D57H, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
8 | - |
D-xylose | mutant enzyme K289E, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
10 | - |
D-xylose | mutant enzyme N215D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
15 | - |
D-xylose | mutant enzyme E186Q/N215D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
27 | - |
D-xylose | mutant enzyme K289H, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
33 | - |
D-xylose | recombinant His6-tagged wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
37 | - |
D-xylose | mutant enzyme K289E, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
42 | - |
D-xylose | mutant enzyme N247D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
83 | - |
D-xylose | native wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
548 | - |
D-xylose | mutant enzyme D57H, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | Streptomyces rubiginosus | - |
D-Xylulose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rubiginosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap crude nickel affinity column chromatography | Streptomyces rubiginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | - |
Streptomyces rubiginosus | D-Xylulose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
D-xylose isomerase | - |
Streptomyces rubiginosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
D-xylose | mutant enzyme D57H, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.056 | - |
D-xylose | mutant enzyme E186Q/N215D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.064 | - |
D-xylose | mutant enzyme K289H, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.07 | - |
D-xylose | mutant enzyme K289E, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.074 | - |
D-xylose | mutant enzyme N247D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.11 | - |
D-xylose | mutant enzyme N247D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.14 | - |
D-xylose | recombinant His12-tagged wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.15 | - |
D-xylose | mutant enzyme Q256D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.18 | - |
D-xylose | mutant enzyme D57H, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.19 | - |
D-xylose | mutant enzyme N215D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.31 | - |
D-xylose | recombinant His6-tagged wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.33 | - |
D-xylose | mutant enzyme D57N, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.41 | - |
D-xylose | native wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.51 | - |
D-xylose | mutant enzyme K289E, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.66 | - |
D-xylose | mutant enzyme E186Q/N215D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.8 | - |
D-xylose | recombinant His12-tagged wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.96 | - |
D-xylose | mutant enzyme D57N, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
1.22 | - |
D-xylose | recombinant His6-tagged wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
1.38 | - |
D-xylose | mutant enzyme K289H, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
2.3 | - |
D-xylose | mutant enzyme N215D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
5.52 | - |
D-xylose | native wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
D-xylose | mutant enzyme D57H, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.0018 | - |
D-xylose | mutant enzyme N247D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.0019 | - |
D-xylose | mutant enzyme K289E, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.0024 | - |
D-xylose | mutant enzyme K289H, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.0037 | - |
D-xylose | mutant enzyme E186Q/N215D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.0049 | - |
D-xylose | native wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.007 | - |
D-xylose | mutant enzyme D57N, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.0094 | - |
D-xylose | recombinant His6-tagged wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.01 | - |
D-xylose | recombinant His12-tagged wild type enzyme, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.018 | - |
D-xylose | mutant enzyme N247D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.019 | - |
D-xylose | mutant enzyme N215D, in 100 mM MES-NaOH (pH 5.8), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.023 | - |
D-xylose | mutant enzyme Q256D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.024 | - |
D-xylose | mutant enzyme D57H, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.059 | - |
D-xylose | mutant enzyme E186Q/N215D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.064 | - |
D-xylose | mutant enzyme K289E, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.181 | - |
D-xylose | mutant enzyme D57N, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.211 | - |
D-xylose | recombinant His12-tagged wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.24 | - |
D-xylose | recombinant His6-tagged wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.321 | - |
D-xylose | mutant enzyme K289H, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
0.676 | - |
D-xylose | mutant enzyme N215D, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus | |
1.84 | - |
D-xylose | native wild type enzyme, in 100 mM HEPES-NaOH (pH 7.7), 20 mM MgCl2, at 25°C | Streptomyces rubiginosus |