5.3.1.5: xylose isomerase

This is an abbreviated version!
For detailed information about xylose isomerase, go to the full flat file.

Reaction

Synonyms

D-XI, D-xylose aldose-ketose-isomerase, D-xylose isomerase, D-Xylose ketoisomerase, D-xylose ketol isomerase, D-xylose ketol-isomerase, D-xylose: ketol-isomerase, D-xylulose keto-isomerase, glucose isomerase, glucose/xylose isomerase, GXI, Isomerase, xylose, Maxazyme, Optisweet, SDXyI, Spezyme, Sweetase, Sweetzyme, Sweetzyme Q, Swetase, T80 xylose isomerase, TcaXI, TNXI, TthXI, XI, XYLA, XylC, xylose (glucose) isomerase, xylose isomerase

ECTree

     5 Isomerases

         5.3 Intramolecular oxidoreductases

             5.3.1 Interconverting aldoses and ketoses, and related compounds

                5.3.1.5 xylose isomerase

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Results	in table
4	Activating Compound
16677	AA Sequence
36	Application
1	CAS Registry Number
62	Cloned(Commentary)
34	Crystallization (Commentary)
2	Expression
3	General Information
9	General Stability
108	Inhibitors
44	kcat/KM [mM/s]
5	Ki Value [mM]
168	KM Value [mM]
2	Localization
124	Metals/Ions
66	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
4	Organic Solvent Stability
360	Organism
6	Pathway
335	PDB ID
56	pH Optimum
22	pH Range
23	pH Stability
1	pI Value
2	Posttranslational Modification
100	Protein Variants
60	Purification (Commentary)
1	Reaction
44	Reaction Type
237	Reference
2	Source Tissue
98	Specific Activity [micromol/min/mg]
2	Storage Stability
355	Substrates and Products (Substrate)
55	Subunits
130	Synonyms
1	Systematic Name
46	Temperature Optimum [°C]
18	Temperature Range [°C]
56	Temperature Stability [°C]
117	Turnover Number [1/s]

Organic Solvent Stability

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Organic Solvent Stability on EC 5.3.1.5 - xylose isomerase

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ORGANIC SOLVENT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Acetone

Streptomyces rubiginosus

-

70-80% of cross-linked crystalline xylose isomerase activity is left after incubation for 24 h at 50°C in buffer solutions (pH 7.2) containing 10-90% acetone. Soluble xylose isomerase is considerably less stable in acetone-containing solutions. The addition of acetone enhances the production of fructose from glucose by enhancing the reaction rate and shifting the equilibrium toward fructose. However, xylose isomerase must be in the form of cross-linked crystals for maximal activity and stability

661385

Ethanol

Streptomyces rubiginosus

-

in buffer containing 50% ethanol only 2% of the initial cross-linked crystalline xylose isomerase activity is retained after 24 h at 50°C. Soluble xylose isomerase is considerably less stable in ethanol-containing solutions

661385

Methanol

Streptomyces rubiginosus

-

cross-linked crystalline xylose isomerase is inactivated in 24 h at 50°C even more in maleate buffer containing 50% methanol

661385

Pyridine

Streptomyces rubiginosus

-

cross-linked crystalline xylose isomerase is inactivated in 24 h at 50°C even more in maleate buffer containing 10% pyridine

661385