Application | Comment | Organism |
---|---|---|
synthesis | production of high fructose corn syrup | Streptomyces rubiginosus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces rubiginosus |
Protein Variants | Comment | Organism |
---|---|---|
D163N/E167Q | 40-60% of wild-type activity, lower pH optimum than wild-type, nearly same tehrmostability as wild-type | Streptomyces rubiginosus |
D56N | turnover number increased by 30-40% over that ofwild-type at pH 7.3, lower pH optimum than wild-type, nearly same thermostability as wild-type | Streptomyces rubiginosus |
D65A | 40-60% of wild-type activity, lower pH optimum than wild-type, nearly same tehrmostability as wild-type | Streptomyces rubiginosus |
D81A | 40-60% of wild-type activity, lower pH optimum than wild-type, nearly same tehrmostability as wild-type | Streptomyces rubiginosus |
E221A | turnover number increased by 30-40% over that from wild-type at pH 7.3, lower pH optimum than wild-type, nearly same tehrmostability as wild-type | Streptomyces rubiginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
D-glucose | 60°C, pH 7.3, with Co2+, wild-type | Streptomyces rubiginosus | |
180 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D81A and wild-type | Streptomyces rubiginosus | |
190 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D65A | Streptomyces rubiginosus | |
200 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D163N/E167Q | Streptomyces rubiginosus | |
310 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutants E221A and D56N | Streptomyces rubiginosus | |
340 | - |
D-glucose | 60°C, pH 7.3, with Co2+, mutant E221A | Streptomyces rubiginosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Streptomyces rubiginosus | |
Mg2+ | - |
Streptomyces rubiginosus | |
Mn2+ | - |
Streptomyces rubiginosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | Streptomyces rubiginosus | part of xylose metabolism | D-Xylulose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rubiginosus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose | - |
Streptomyces rubiginosus | D-Fructose | - |
r | |
D-Xylose | - |
Streptomyces rubiginosus | D-Xylulose | - |
r | |
D-Xylose | part of xylose metabolism | Streptomyces rubiginosus | D-Xylulose | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | 0.55 | D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant E221A | Streptomyces rubiginosus | |
0.031 | 0.51 | D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D56N | Streptomyces rubiginosus | |
0.04 | 1.97 | D-glucose | 60°C, pH 7.3, with Co2+, mutant E221A | Streptomyces rubiginosus | |
3 | 6 | D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D163N/E167Q | Streptomyces rubiginosus | |
3 | 6 | D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D65A | Streptomyces rubiginosus | |
3 | 6 | D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D81A | Streptomyces rubiginosus | |
3.02 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D163N/E167Q | Streptomyces rubiginosus | |
3.3 | - |
D-glucose | 60°C, pH 7.3, with Co2+, wild-type | Streptomyces rubiginosus | |
3.3 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, wild-type | Streptomyces rubiginosus | |
3.37 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D65A | Streptomyces rubiginosus | |
3.41 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D81A | Streptomyces rubiginosus | |
4.53 | - |
D-glucose | 60°C, pH 7.3, with Co2+, wild-type | Streptomyces rubiginosus | |
5.27 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, wild-type | Streptomyces rubiginosus | |
6.99 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant E221A | Streptomyces rubiginosus | |
7.53 | - |
D-glucose | 60°C, pH 7.3, with Mg2+ and Co2+, mutant D56N | Streptomyces rubiginosus | |
9.22 | - |
D-glucose | 60°C, pH 7.3, with Co2+, mutant E221A | Streptomyces rubiginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in presence of Co2+, mutant E221A | Streptomyces rubiginosus |
8 | - |
in presence of Mg2+ and Co2+, mutants E221A and D56N | Streptomyces rubiginosus |
8 | 8.2 | in presence of Mg2+ and Co2+, mutants D65A and D163N/E167Q | Streptomyces rubiginosus |
8.1 | 8.3 | in presence of Mg2+ and Co2+, mutant D81A | Streptomyces rubiginosus |
8.5 | - |
in presence of Mg2+ and Co2+, wild-type | Streptomyces rubiginosus |
8.8 | - |
in presence of Co2+, wild-type | Streptomyces rubiginosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Streptomyces rubiginosus |