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4.6.1.13: phosphatidylinositol diacylglycerol-lyase

This is an abbreviated version!
For detailed information about phosphatidylinositol diacylglycerol-lyase, go to the full flat file.

Word Map on EC 4.6.1.13

Reaction

1-phosphatidyl-1D-myo-inositol
=
1D-myo-inositol 1,2-cyclic phosphate
 +
1,2-diacyl-sn-glycerol

Synonyms

1-phosphatidyl-D-myo-inositol inositolphosphohydrolase (cyclic-phosphate-forming), 1-phosphatidylinositol phosphodiesterase, EC 3.1.4.10, monophosphatidylinositol phosphodiesterase, More, Phosphatidylinositol diacylglycerol-lyase, phosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphatidylinositol-specific phospholipase C, phosphatidylinositol-specific PLC, phosphatidylinositolphospholipase C, PI-phospholipase C, PI-PLC, PLC, PLC1, PLC2, PLC3, PLC4, PLC5, PLC6

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.13 phosphatidylinositol diacylglycerol-lyase

Cloned

Cloned on EC 4.6.1.13 - phosphatidylinositol diacylglycerol-lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the L4440 vector, transformation of plasmids into Escherichia coli HT115DE3
-
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
expressed in Saccharomyces cerevisiae strain BY4741
-
expression in Escherichia coli MM294
-
expression in Escherichia coli, construction of four vectors for high-level expression
expression in Listeria monocytogenes. Listeria monocytogenes expressing Bacillus anthracis phosphatidylinositol-specific phospholipase C shows significantly decreased efficiencies of ascape from a phagosome and in cell-to-cell spread
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-Codonplus (DE3)-RIL
inserted into the pTYB11 N-terminal fusion vector between Sap I and EcoR I restriction sites. Recombinant plasmid, IMPACT-Y, transformed into Escherichia coli ER2566 cells for overexpression
-
overexpressed in Escherichia coli
-
overexpression in Escherichia coli BL21
-
PI-PLC gene is part of the virulence gene cluster, overexpression in Escherichia coli MM294
-
transformed into Escherichia coli BL21-Codonplus (DE3)-RIL cells
wild-type and mutant PI-PLC, expression in Escherichia coli BL21
-
wild-type and mutants overexpressed in Escherichia coli