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4.6.1.13: phosphatidylinositol diacylglycerol-lyase

This is an abbreviated version!
For detailed information about phosphatidylinositol diacylglycerol-lyase, go to the full flat file.

Word Map on EC 4.6.1.13

Reaction

1-phosphatidyl-1D-myo-inositol
=
1D-myo-inositol 1,2-cyclic phosphate
 +
1,2-diacyl-sn-glycerol

Synonyms

1-phosphatidyl-D-myo-inositol inositolphosphohydrolase (cyclic-phosphate-forming), 1-phosphatidylinositol phosphodiesterase, EC 3.1.4.10, monophosphatidylinositol phosphodiesterase, More, Phosphatidylinositol diacylglycerol-lyase, phosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphatidylinositol-specific phospholipase C, phosphatidylinositol-specific PLC, phosphatidylinositolphospholipase C, PI-phospholipase C, PI-PLC, PLC, PLC1, PLC2, PLC3, PLC4, PLC5, PLC6

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.13 phosphatidylinositol diacylglycerol-lyase

Specific Activity

Specific Activity on EC 4.6.1.13 - phosphatidylinositol diacylglycerol-lyase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.2
mutant Y246S/Y247S/Y248S, with 8 mM cIP as substrate
0.4
mutant Y246S/Y247S/Y248S/Y251S, with 8 mM cIP as substrate
0.6
mutant Y247S/Y251S, with 8 mM cIP as substrate
0.8
mutant Y246S/Y247S/Y248S/Y251S, with 8 mM cIP as substrate, in the presence of 5 mM diheptanoylphosphatidylcholine
1.6
mutant Y246S/Y247S/Y248S/Y251S, with phosphatidylinositol as substrate, in the presence of 2 mM POPC (for the small unilamellar vesicles)
112
mutant Y246S/Y247S/Y248S/Y251S, with phosphatidylinositol as substrate, in the presence of 32 mM diheptanoylphosphatidylcholine
1630
-
wild-type, for phosphatidylinositol/diC7-phosphatidylcholine, in 50 mM HEPES buffer, pH 7.5, with 1 mM EDTA, 5 mM dithiothreitol, and 0.1 mg/ml bovine serum albumin, at 28°C
2.1
mutant Y246S/Y247S/Y248S, with phosphatidylinositol as substrate, in the presence of 2 mM POPC (for the small unilamellar vesicles)
2.3
wild-type, with 8 mM cIP as substrate
2.9
mutant Y246S/Y247S/Y248S, with 8 mM cIP as substrate, in the presence of 5 mM diheptanoylphosphatidylcholine
239
mutant Y247S/Y251S, with phosphatidylinositol as substrate, in the presence of 16 mM Triton X-100
30
-
pH 7.2, phosphatidylinositol as substrate, in absence of MgCl2
301
mutant Y246S/Y247S/Y248S, with phosphatidylinositol as substrate, in the presence of 32 mM diheptanoylphosphatidylcholine
3560
-
pH 7.5, 37°C, wild-type PI-PLC, in absence of Ca2+
375
wild-type, with phosphatidylinositol as substrate, in the presence of 16 mM Triton X-100
401
-
W47I mutant PI-PLC, phosphotransferase activity
41
mutant Y247S/Y251S, with 8 mM cIP as substrate, in the presence of 5 mM diheptanoylphosphatidylcholine
556
-
wild-type PI-PLC, phosphotransferase activity
558
-
W47F mutant PI-PLC, phosphotransferase activity
560
wild-type, with phosphatidylinositol as substrate, in the presence of 32 mM diheptanoylphosphatidylcholine
6.7
mutant Y247S/Y251S, with phosphatidylinositol as substrate, in the presence of 2 mM POPC (for the small unilamellar vesicles)
62
mutant Y246S/Y247S/Y248S/Y251S, with phosphatidylinositol as substrate, in the presence of 16 mM Triton X-100
658
-
W242F mutant PI-PLC, phosphotransferase activity
670
mutant Y247S/Y251S, with phosphatidylinositol as substrate, in the presence of 32 mM diheptanoylphosphatidylcholine
684
-
W242I mutant PI-PLC, phosphotransferase activity
700 - 1300
-
pH 7.2, phosphatidylinositol as substrate, in presence of MgCl2
73
wild-type, with 8 mM cIP as substrate, in the presence of 5 mM diheptanoylphosphatidylcholine
9.5
wild-type, with phosphatidylinositol as substrate, in the presence of 2 mM POPC (for the small unilamellar vesicles)
98
mutant Y246S/Y247S/Y248S, with phosphatidylinositol as substrate, in the presence of 16 mM Triton X-100
additional information