4.6.1.13: phosphatidylinositol diacylglycerol-lyase
This is an abbreviated version!
For detailed information about phosphatidylinositol diacylglycerol-lyase, go to the full flat file.
Word Map on EC 4.6.1.13
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4.6.1.13
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11beta-hydroxysteroid
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cortisone
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phosphatidylcholine-specific
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11beta-hsd2
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pc-plc
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molecular biology
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analysis
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medicine
- 4.6.1.13
- 11beta-hydroxysteroid
- cortisone
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phosphatidylcholine-specific
- 11beta-hsd2
- pc-plc
- molecular biology
- analysis
- medicine
Reaction
Synonyms
1-phosphatidyl-D-myo-inositol inositolphosphohydrolase (cyclic-phosphate-forming), 1-phosphatidylinositol phosphodiesterase, EC 3.1.4.10, monophosphatidylinositol phosphodiesterase, More, Phosphatidylinositol diacylglycerol-lyase, phosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphatidylinositol-specific phospholipase C, phosphatidylinositol-specific PLC, phosphatidylinositolphospholipase C, PI-phospholipase C, PI-PLC, PLC, PLC1, PLC2, PLC3, PLC4, PLC5, PLC6
ECTree
4.6.1.13 phosphatidylinositol diacylglycerol-lyaseAdvanced search results
results (
results (Activating Compound
Activating Compound on EC 4.6.1.13 - phosphatidylinositol diacylglycerol-lyase
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ACTIVATING COMPOUND 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
butyl-fluorescein myo-inositol phosphate
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two molecules bind to enzyme, one at the active site and one at a subsite, causing an increase in activity, kinetics
dihexanoyl phosphatidylcholine
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activates, 4-5fold increase in catalytic efficiency, binds to a lipid-binding subsite, not to the active site, maximal activation at 0.4 mM
dihexanoylphosphatidylcholine
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non-substrate activator lipid, maximum PI-PLC activity at 0.7-1 mM
dimethylformamide
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water-miscible, enhances phosphotransferase activity
Dimethylsulfoxide
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water-miscible, enhances phosphotransferase activity
heme
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heme receptor mediates the stimulatory effect of heme on the (Na+ + K+)ATPase activity through a PIPLC/PKC signaling pathway
KCl
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ionic strength, and not the salt identity, is important for PI-PLC activation towards phosphatidylinositol in micelles. Added salt has a synergistic effect with zwitterionic phospholipids, leading to high specific activities for phosphatidylinositol cleavage with only moderate dilution of the anionic substrate in the interface. This kinetic activation correlates with weakening of strong PI-PLC hydrophobic interactions with the interface. PI-PLC cleavage of phosphatidylinositol presented in small unilamellar vesicles is activated by salt
phosphatidic acid
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binding to nonsubstrate anionic interfaces enhances the catalytic activity of PI-PLC, interfacial binding studies, activation mechanism
phosphatidylglycerol
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binding to nonsubstrate anionic interfaces enhances the catalytic activity of PI-PLC, interfacial binding studies, activation mechanism
phosphatidylmethanol
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binding to nonsubstrate anionic interfaces enhances the catalytic activity of PI-PLC, interfacial binding studies, activation mechanism
phosphatidylserine
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binding to nonsubstrate anionic interfaces enhances the catalytic activity of PI-PLC, interfacial binding studies, activation mechanism
diacylglycerol
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activates, increases the hydrolytic activity of PI-PLC on large unilamellar vesicles containing 5-40% phosphatidylinositol
Isopropanol
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water-miscible, maximum activation at 30%, activates regardless of the type of phosphatidylinositol substrate, enhances phosphotransferase activity
phosphatidylcholine
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binding to nonsubstrate zwitterionic phosphatidylcholine interfaces enhances the catalytic activity of PI-PLC, interfacial binding studies, activation mechanism
phosphatidylcholine
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PI-PLC is activated by nonsubstrate interfaces such as phosphatidylcholine micelles or bilayers, activation corresponds with partial insertion into the interface of Trp-47 and Trp-242 in the rim of the alphabeta-barrel
additional information
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isopropanol and diheptanoylphosphatidylcholine activate the hydrolytic activity towards 1D-myo-inositol 1,2-cyclic phosphate, PI-PLC exhibits kinetic interfacial activation
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additional information
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PI-PLC exhibits several types of kinetic interfacial activation by interfaces, roles of Trp-47 and Trp-242
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additional information
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for small unilamellar vesicles containing anionic lipids and phosphatidylcholine, PI-PLC binding is strengthened by even small amounts of phosphatidylcholine
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