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Literature summary for 4.6.1.13 extracted from

  • Chen, W.; Goldfine, H.; Ananthanarayanan, B.; Cho, W.; Roberts, M.
    Listeria monocytogenes phosphatidylinositol-specific phospholipase C: Kinetic activation and homing in on different interfaces (2009), Biochemistry, 48, 3578-3592.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
KCl ionic strength, and not the salt identity, is important for PI-PLC activation towards phosphatidylinositol in micelles. Added salt has a synergistic effect with zwitterionic phospholipids, leading to high specific activities for phosphatidylinositol cleavage with only moderate dilution of the anionic substrate in the interface. This kinetic activation correlates with weakening of strong PI-PLC hydrophobic interactions with the interface. PI-PLC cleavage of phosphatidylinositol presented in small unilamellar vesicles is activated by salt Listeria monocytogenes

Cloned(Commentary)

Cloned (Comment) Organism
inserted into the pTYB11 N-terminal fusion vector between Sap I and EcoR I restriction sites. Recombinant plasmid, IMPACT-Y, transformed into Escherichia coli ER2566 cells for overexpression Listeria monocytogenes

Protein Variants

Protein Variants Comment Organism
F237A most approaches wild-type PI-PLC in its dependence on enzyme concentration Listeria monocytogenes
F237W even at high concentrations, has high specific activity comparable to dilute unaltered recombinant PI-PLC, does not form the aggregates with anionic lipid-rich vesicles that are disrupted by excess detergent and salt, although it is still activated to about the same extent as wild-type by salt Listeria monocytogenes
L151A added KCl (0.15 M) still enhances PI-PLC cleavage of phosphatidylinositol in TX-100 micelles, although KCl effects are much more modest (1.6fold increase) compared to wild-type, F237A or F237W Listeria monocytogenes
L235A added KCl (0.15 M) still enhances PI-PLC cleavage of phosphatidylinositol in TX-100 micelles, although KCl effects are much more modest (1.4fold increase) compared to wild-type, F237A or F237W Listeria monocytogenes
W49A added KCl (0.15 M) still enhances PI-PLC cleavage of phosphatidylinositol in TX-100 micelles, although KCl effects are much more modest (1.8fold increase) compared to wild-type, F237A or F237W Listeria monocytogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
1 * 28000, in solution, laser light scattering Listeria monocytogenes
28200
-
laser light scattering Listeria monocytogenes
33000
-
sequence analysis Listeria monocytogenes

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by sonication and centrifugation, on chitin column and by gel filtration, more than 85% pure Listeria monocytogenes

Storage Stability

Storage Stability Organism
4°C, 1 month Listeria monocytogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dibutyrylphosphatidylinositol is a poor substrate, necessitating long incubation times (2 to 5 h) if the same enzyme concentration is to be used in the absence and presence of salts and amphiphiles Listeria monocytogenes 1D-myo-inositol 1,2-(cyclic)-phosphate + 1,2-dibutyryl-sn-glycerol
-
?
additional information Phe237 is a key residue in the very tight binding of PI-PLC to membranes containing anionic phospholipids Listeria monocytogenes ?
-
?
phosphatidylinositol
-
Listeria monocytogenes diacylglycerol + myo-inositol 1,2-cyclic phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28000, in solution, laser light scattering Listeria monocytogenes

Synonyms

Synonyms Comment Organism
phosphatidylinositol-specific phospholipase C
-
Listeria monocytogenes
PI-PLC
-
Listeria monocytogenes