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4.3.3.6: pyridoxal 5'-phosphate synthase (glutamine hydrolysing)

This is an abbreviated version!
For detailed information about pyridoxal 5'-phosphate synthase (glutamine hydrolysing), go to the full flat file.

Word Map on EC 4.3.3.6

Reaction

D-ribose 5-phosphate
+
D-glyceraldehyde 3-phosphate
 +
L-glutamine
=
pyridoxal 5'-phosphate
 +
L-glutamate
 + 3 H2O +
phosphate

Synonyms

Pdx1, PDX2, PdxS, Ph1355, PLP synthase, PLP-synthase complex, PLPS, pyridoxal 5'-phosphate synthase, pyridoxal 5-phosphate synthase, pyridoxal 5-phosphate synthase Snz1, pyridoxal biosynthesis lyase, pyridoxal biosynthesis lyase PdxS, Rv2606c, Snz1

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.3 Amine-lyases
                4.3.3.6 pyridoxal 5'-phosphate synthase (glutamine hydrolysing)

Cloned

Cloned on EC 4.3.3.6 - pyridoxal 5'-phosphate synthase (glutamine hydrolysing)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta2 (DE3) pLysS
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3)
gene btrC2, DNA and amino acid sequence determination, genetic organization, gene btrC2 is not found in the butirosin-biosynthestic gene cluster, sequence comparison, gene complementation of a btrC2 disruptant by Bacillus subtilis gene pdxT, recombinant expression of untagged BtrC2 in Escherichia coli
gene lmo2101 or pdxS, encoding a subunit of pyridoxal 5'-phosphate synthase, quantitative PCR expression analysis, determination of transcription start points of genes pdxS and pdxR, overview
gene pdxS, DNA and amino acid sequence determination, genetic organization, recombinant expression of untagged protein encoded by pdxS in Escherichia coli
gene Rv2606c, recombinant expression of N-terminally His6-tagged enzyme with a TEV protease cleavage site in Escherichia coli strain B834
genes pdxS and pdxT, genetic organization, expression of His-tagged PdxT in Escherichia coli
-
His-tagged protein expressed in Escherichia coli BL21 (DE3)
His-tagged protein expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL
His-tagged subunits Pdx1 and Pdx2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL
overexpressed in Escherichia coli strain Rosetta2 (DE3)
overexpression in Escherichia coli
Pdx1 and Pdx2 with and without His-tag expressed
-
Strep-Tag-fusion proteins expressed in Escherichia coli BLR (DE3)
-