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Literature summary for 4.3.3.6 extracted from

  • Moccand, C.; Kaufmann, M.; Fitzpatrick, T.
    It takes two to Tango: Defining an essential second active site in pyridoxal 5'-phosphate synthase (2011), PLoS ONE, 6, e16042.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.3.6

Cloned(Commentary)

Cloned (Comment) Organism
Pdx1 and Pdx2 with and without His-tag expressed Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
E105D Pdx1 Bacillus subtilis
H115A Pdx1 Bacillus subtilis
H115A/R138A Pdx1 Bacillus subtilis
H170N Pdx2, catalytically inert Bacillus subtilis
K187A Pdx1, 84% of wild-type activity Bacillus subtilis
R138A Pdx1 Bacillus subtilis
R288A Pdx1, able to activate Pdx2 Bacillus subtilis
R288K Pdx1, able to activate Pdx2 Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.032
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.035
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.04
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.04
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.043
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.043
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.044
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.122
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.155
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.186
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.195
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.22
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.229
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.249
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.262
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.267
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.307
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.93
-
 L-glutamine Pdx2 glutaminase activity, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
1.12
-
 L-glutamine Pdx2 glutaminase activity, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
1.16
-
 L-glutamine Pdx2 glutaminase activity, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
1.16
-
 L-glutamine Pdx2 glutaminase activity, wild-type protein, pH 7.5, 37°C Bacillus subtilis
1.2
-
 L-glutamine Pdx2 glutaminase activity, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
1.31
-
 L-glutamine Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
1.33
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
1.44
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
1.54
-
 L-glutamine Pdx2 glutaminase activity, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32830
-
 unmodified Pdx1, ESI-MS Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine in the presence of Pdx1 and Pdx2 Bacillus subtilis pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
pyridoxal 5'-phosphate synthase
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0001
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.00013
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0002
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0002
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0005
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0005
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.00055
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.0006
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.00062
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.022
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.024
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.024
-
 L-glutamine Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.024
-
 L-glutamine Pdx2 glutaminase activity, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.024
-
 L-glutamine Pdx2 glutaminase activity, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.026
-
 L-glutamine Pdx2 glutaminase activity, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0295
-
 L-glutamine Pdx2 glutaminase activity, wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.0305
-
 L-glutamine Pdx2 glutaminase activity, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0307
-
 L-glutamine Pdx2 glutaminase activity, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00033
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.00058
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.00077
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.00093
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0019
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0023
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0023
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.003
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.0032
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0038
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0039
-
 glyceraldehyde 3-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.0041
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0046
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0058
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.015
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.0154
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.016
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.016
-
 L-glutamine Pdx2 glutaminase activity, His-tagged R138A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.017
-
 L-glutamine Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0172
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.018
-
 L-glutamine Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.0192
-
 D-ribose 5-phosphate pyridoxal 5'-phosphate formation, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.021
-
 L-glutamine Pdx2 glutaminase activity, His-tagged wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.024
-
 L-glutamine Pdx2 glutaminase activity, His-tagged E105D mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.026
-
 L-glutamine Pdx2 glutaminase activity, R288A mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis
0.026
-
 L-glutamine Pdx2 glutaminase activity, wild-type protein, pH 7.5, 37°C Bacillus subtilis
0.033
-
 L-glutamine Pdx2 glutaminase activity, R288K mutant Pdx1 protein, pH 7.5, 37°C Bacillus subtilis