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Literature summary for 4.3.3.6 extracted from

  • Derrer, B.; Windeisen, V.; Guedez Rodriguez, G.; Seidler, J.; Gengenbacher, M.; Lehmann, W.; Rippe, K.; Sinning, I.; Tews, I.; Kappes, B.
    Defining the structural requirements for ribose 5-phosphate-binding and intersubunit cross-talk of the malarial pyridoxal 5-phosphate synthase (2010), FEBS Lett., 584, 4169-4174.
    View publication on PubMed
Search for more literature for 4.3.3.6

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
DELTA 270-301 mainly monomer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis Plasmodium falciparum
DELTA 273-301 dodecamer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis Plasmodium falciparum
DELTA 279-301 dodecamer, Pdx2 activation, reduced pyridoxal 5'-phosphate synthesis Plasmodium falciparum
DELTA 287-301 dodecamer, Pdx2 activation, precipitates upon addition of glyceraldehyd 3-phosphate Plasmodium falciparum
DELTA 293-301 behaviour like DELTA 287-301 Plasmodium falciparum
DELTA 295-301 behaviour like DELTA 287-301 Plasmodium falciparum
S270A/DELTA 273-301 dodecamer Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 12 * 29000, two interacting hexamers, analytical ultracentrifugation, 35320.7 Da determined by ESI-MS Plasmodium falciparum
363000
-
 analytical ultracentrifugation, 420000 calculated Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum C6KT50
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+), gel filtration Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00025
-
 DELTA 279-301 protein, in the presence of Pdx2, pH 8.0, 37°C Plasmodium falciparum
0.000779
-
 wild-type protein, in the presence of Pdx2, pH 8.0, 37°C Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine in the presence of Pdx2 Plasmodium falciparum pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
 ?
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+ in the absence of Pdx2 Plasmodium falciparum pyridoxal 5'-phosphate + 4 H2O + phosphate
-
 ?

Subunits

Subunits Comment Organism
dodecamer 12 * 29000, two interacting hexamers, analytical ultracentrifugation, 35320.7 Da determined by ESI-MS Plasmodium falciparum

Synonyms

Synonyms Comment Organism
pyridoxal 5-phosphate synthase complex of Pdx1 and Pdx2 Plasmodium falciparum