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Literature summary for 4.3.3.6 extracted from
- Overexpression, crystallization and preliminary X-ray crystallographic analysis of pyridoxal biosynthesis lyase PdxS from Pyrococcus horikoshii (2012), Acta Crystallogr. Sect. F, 68, 440-442.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta2 (DE3) pLysS | Pyrococcus horikoshii |
| overexpression in Escherichia coli | Pyrococcus horikoshii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallization at 22°C using 2-methyl-2,4-pentanediol as a precipitant. Crystals of PdxS diffract to 2.61 A resolution and belong to the monoclinic space group P2(1), with unit-cell parameters a = 59.30, b = 178.56, c = 109.23 A, beta = 102.97°. The asymmetric unit contained six monomers | Pyrococcus horikoshii |
| hanging-drop vapour-diffusion method, 296 K, 2-methyl-2,4-pentanediol | Pyrococcus horikoshii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37014 | - |
6 * 37014, calculated, dynamic light-scattering analysis | Pyrococcus horikoshii |
| 200000 | - |
dynamic light-scattering analysis | Pyrococcus horikoshii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | - |
- |
- |
| Pyrococcus horikoshii | O59080 | - |
- |
| Pyrococcus horikoshii OT-3 | O59080 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion exchange chromatography (Q Sepharose), gel filtration | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ribulose 5-phosphate + glyceraldehyde 3-phosphate + NH3 | - |
Pyrococcus horikoshii | pyridoxal 5'-phosphate + 4 H2O + phosphate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | 6 * 37014, calculated, dynamic light-scattering analysis | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PdxS | - |
Pyrococcus horikoshii |
| PdxS | PdxS catalyses the condensation of ribulose 5-phosphate, glyceraldehyde 3-phosphate and ammonia, while glutamine amidotransferase (PdxT) catalyses the production of ammonia from glutamine. PdxS and PdxT form a complex | Pyrococcus horikoshii |
| Ph1355 | - |
Pyrococcus horikoshii |
| pyridoxal biosynthesis lyase | - |
Pyrococcus horikoshii |
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