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4.2.3.23: germacrene-A synthase

This is an abbreviated version!
For detailed information about germacrene-A synthase, go to the full flat file.

Word Map on EC 4.2.3.23

Reaction

(2E,6E)-farnesyl diphosphate
=
(+)-(R)-gemacrene A
 +
diphosphate

Synonyms

(+)-(10R)-germacrene A synthase, (+)-germacrene A synthase, FENS, GAS, GAS1, GAS2, GASlo, GASsh, germacrene A synthase, germacrene A synthase 1, germacrene A synthase 2, guaiadiene synthase, NS1, Sc1, sesquiterpene cyclase, sesquiterpene synthase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.23 germacrene-A synthase

Cloned

Cloned on EC 4.2.3.23 - germacrene-A synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from a constructed glandular trichome cDNA library, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression in Escherichia coli strain BL21-DE3-RIL
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli
DNA library construction, phage display, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli as N-terminal thioredoxin fusion protein
DNA sequence determination and analysis
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Saccharomyces cerevisiae EPY300 cells
expression in Escherichia coli
expression in Escherichia coli, N-terminal thioredoxin fusion protein
expression of wild-type and mutant enzymes in bacteria
NS1 is overexpressed at high levels in Escherichia coli. The gene encoding NS1 is flanked by an ORF encoding a putative cytochrome P450 monooxygenase
-
thioredoxin-fusion protein expressed in Escherichia coli