4.2.3.23: germacrene-A synthase

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For detailed information about germacrene-A synthase, go to the full flat file.

Reaction

Synonyms

(+)-(10R)-germacrene A synthase, (+)-germacrene A synthase, FENS, GAS, GAS1, GAS2, GASlo, GASsh, germacrene A synthase, germacrene A synthase 1, germacrene A synthase 2, guaiadiene synthase, NS1, Sc1, sesquiterpene cyclase, sesquiterpene synthase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.23 germacrene-A synthase

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Results	in table
1	Activating Compound
32	AA Sequence
2	Application
1	CAS Registry Number
14	Cloned(Commentary)
1	Cofactor
1	Expression
1	General Stability
2	Inhibitors
2	kcat/KM [mM/s]
18	KM Value [mM]
4	Localization
5	Metals/Ions
11	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
21	Organism
7	Pathway
10	pH Optimum
1	pH Range
4	pI Value
1	Posttranslational Modification
14	Protein Variants
8	Purification (Commentary)
3	Reaction
19	Reference
29	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
34	Substrates and Products (Substrate)
15	Subunits
31	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
8	Turnover Number [1/s]

Purification

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Purification on EC 4.2.3.23 - germacrene-A synthase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

201fold from roots by anion exchange and dye-ligand affinity chromatography

Cichorium intybus

-

663110

cell debris is cleared by centrifugation and the cleared protein extract is used to purify proteins by metal-affinity chromatography

Nostoc sp.

-

692875

Ni-NTA affinity chromatography

Helianthus annuus

B0FGA9, Q4U3F7

702923

partially, recombinant enzyme

Ixeridium dentatum

-

662712

recombinant long isozyme from Escherichia coli, native isozyme partially from chicory heads by anion exchange chromatography

Cichorium intybus

Q8LSC2, Q8LSC3

663116

recombinant short isozyme from Escherichia coli, native isozyme partially from chicory heads by anion exchange chromatography

Cichorium intybus

Q8LSC2, Q8LSC3

663116

recombinant thioredoxin fusion enzyme from Escherichia coli by nickel chelate affinity chromatography

Solidago canadensis

Q9AR67

663040

recombinant wild-type and mutant enzymes from bacteria

Ixeridium dentatum

Q6YN71

661381