4.2.3.23: germacrene-A synthase
This is an abbreviated version!
For detailed information about germacrene-A synthase, go to the full flat file.
Word Map on EC 4.2.3.23
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4.2.3.23
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sesquiterpene
-
terpene
-
lactones
-
farnesyl
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+-germacrene
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asteraceae
-
chicory
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cichorium
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annua
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costunolide
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cynaropicrin
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feverfew
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cynara
-
cardunculus
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intybus
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parthenolide
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amorpha-4,11-diene
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parthenium
-
tanacetum
-
analysis
- 4.2.3.23
-
sesquiterpene
-
terpene
- lactones
-
farnesyl
-
+-germacrene
- asteraceae
- chicory
-
cichorium
- annua
- costunolide
- cynaropicrin
- feverfew
-
cynara
- cardunculus
- intybus
- parthenolide
- amorpha-4,11-diene
- parthenium
-
tanacetum
- analysis
Reaction
Synonyms
(+)-(10R)-germacrene A synthase, (+)-germacrene A synthase, FENS, GAS, GAS1, GAS2, GASlo, GASsh, germacrene A synthase, germacrene A synthase 1, germacrene A synthase 2, guaiadiene synthase, NS1, Sc1, sesquiterpene cyclase, sesquiterpene synthase
ECTree
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Turnover Number
Turnover Number on EC 4.2.3.23 - germacrene-A synthase
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0.13
isoform GAS1, at pH 7.0 and 23°C
0.28
(2E,6E)-farnesyl diphosphate
isoform GAS2, at pH 7.0 and 23°C
0.00035
pH 7.0, 25°C, recombinant mutant T433A
0.00037
2-trans,6-trans-farnesyl diphosphate
pH 7.0, 25°C, recombinant mutant T433A/A435T
0.016
2-trans,6-trans-farnesyl diphosphate
pH 7.0, 25°C, recombinant wild-type enzyme
0.017
2-trans,6-trans-farnesyl diphosphate
pH 7.0, 25°C, recombinant mutant S434A
0.044
2-trans,6-trans-farnesyl diphosphate
pH 7.0, 25°C, recombinant mutant A435T
4.4
2-trans,6-trans-farnesyl diphosphate
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determined by incubating purified enzyme with various concentrations of 1 to 100 microM 2-trans,6-trans-farnesyl diphosphate