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Literature summary for 4.2.3.23 extracted from

  • Kim, M.Y.; Chang, Y.J.; Bang, M.H.; Baek, N.I.; Jin, J.; Lee, C.H.; Kim, S.U.
    cDNA isolation and characterization of (+)-germacrene A synthase from Ixeris dentata form. albiflora Hara (2005), J. Plant Biol., 48, 178-186.
No PubMed abstract available
Search for more literature for 4.2.3.23

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis Ixeridium dentatum

Protein Variants

Protein Variants Comment Organism
additional information deletion of the N-terminal 44 amino acid residues leads to loss of 90% of catalytic activity Ixeridium dentatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11
-
 2-trans,6-trans-farnesyl diphosphate pH 7.5-8.0, 25°C Ixeridium dentatum
14.9
-
 2-trans,6-trans-farnesyl diphosphate pH 7.5-8.0, 37°C Ixeridium dentatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67100
-
 x * 67100, DNA sequence calculation Ixeridium dentatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate Ixeridium dentatum
-
 (+)-(10R)-germacrene A + diphosphate
-
 ?
additional information Ixeridium dentatum (+)-germacrene A synthesis is the committed step in the biosynthesis of bitter sesquiterpene lactones in chicory ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ixeridium dentatum
-
 formerly Ixeris albiflora
-

Purification (Commentary)

Purification (Comment) Organism
partially, recombinant enzyme Ixeridium dentatum

Reaction

Reaction Comment Organism Reaction ID
(2E,6E)-farnesyl diphosphate = (+)-(R)-gemacrene A + diphosphate the catalytic site is located at the N-terminal 44 amino acid residues Ixeridium dentatum
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf expression levels decline near the onset of flowering Ixeridium dentatum
-
root
-
 Ixeridium dentatum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate
-
 Ixeridium dentatum (+)-(10R)-germacrene A + diphosphate
-
 ?
2-trans,6-trans-farnesyl diphosphate
-
 Ixeridium dentatum (+)-(10R)-germacrene A + diphosphate reaction product analysis by GC-MS and NMR ?
additional information (+)-germacrene A synthesis is the committed step in the biosynthesis of bitter sesquiterpene lactones in chicory Ixeridium dentatum ?
-
 ?
additional information no activity with geranyl diphosphate Ixeridium dentatum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 67100, DNA sequence calculation Ixeridium dentatum

Synonyms

Synonyms Comment Organism
(+)-germacrene A synthase
-
 Ixeridium dentatum
GAS
-
 Ixeridium dentatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 recombinant enzyme Ixeridium dentatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 recombinant enzyme Ixeridium dentatum

pI Value

Organism Comment pI Value Maximum pI Value
Ixeridium dentatum DNA sequence calculation
-
 5.2