Cloned (Comment) | Organism |
---|---|
NS1 is overexpressed at high levels in Escherichia coli. The gene encoding NS1 is flanked by an ORF encoding a putative cytochrome P450 monooxygenase | Nostoc sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
2-trans,6-trans-farnesyl diphosphate | in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM mercaptoethanol | Nostoc sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enzyme requires Mg2+ for catalysis, Mg2+ ions bind to two metal-binding motifs that are conserved among all terpene synthases | Nostoc sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | Nostoc sp. | - |
(+)-(10R)-germacrene A + diphosphate | - |
? | |
2-trans,6-trans-farnesyl diphosphate | Nostoc sp. PCC 712 | - |
(+)-(10R)-germacrene A + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc sp. | - |
strain PCC 7120, previously Anabaena sp. | - |
Purification (Comment) | Organism |
---|---|
cell debris is cleared by centrifugation and the cleared protein extract is used to purify proteins by metal-affinity chromatography | Nostoc sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | - |
Nostoc sp. | (+)-(10R)-germacrene A + diphosphate | - |
? | |
2-trans,6-trans-farnesyl diphosphate | - |
Nostoc sp. PCC 712 | (+)-(10R)-germacrene A + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
germacrene A synthase | - |
Nostoc sp. |
NS1 | sesquiterpene synthase | Nostoc sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
2-trans,6-trans-farnesyl diphosphate | determined by incubating purified enzyme with various concentrations of 1 to 100 microM 2-trans,6-trans-farnesyl diphosphate | Nostoc sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Nostoc sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | assay at | Nostoc sp. |