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4.1.99.12: 3,4-dihydroxy-2-butanone-4-phosphate synthase

This is an abbreviated version!
For detailed information about 3,4-dihydroxy-2-butanone-4-phosphate synthase, go to the full flat file.

Word Map on EC 4.1.99.12

Reaction

D-ribulose 5-phosphate
=
formate
 +
L-3,4-dihydroxybutan-2-one 4-phosphate

Synonyms

3,4-dihydroxy 2-butanone-4-phosphate synthase, 3,4-dihydroxy-2-butanone 4-phosphate synthase, 3,4-dihydroxy-2-butanone-4-phosphate synthase, AtRIBA1, AtRIBA2, bifunctional GCHYII/DHBP, bifunctional GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase, DBPS, DHBP synthase, DHBPS, DHBPS/GCHII, dihydroxybutanone phosphate synthase, L-3,4-dihydroxy-2-butanone 4-phosphate synthase, L-3,4-dihydroxy-2-butanone-4-phosphate synthase, LcRIBA, More, Mtb-DHBPS, Mtb-ribA2, NbRibA, Rib3, ribA, ribA2, ribB, Sp0176, vDHBPS, vribB

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.12 3,4-dihydroxy-2-butanone-4-phosphate synthase

Cloned

Cloned on EC 4.1.99.12 - 3,4-dihydroxy-2-butanone-4-phosphate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning by functional complementation of an Escherichia coli DS knockout mutant, expression in Escherichia coli strain BL21(DE3)
-
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strains XL-1 Blue and M15
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expression in Escherichia coli
functional expression of the synthetic gene in Escherichia coli strains XL1-Blue and M15
gene At2g22450, phylogenetic analysis, recombinant expression of His-tagged N-terminally truncated AtRIBA2 protein in Escherichia coli, recombinant expression fo GFP-tagged isozymes AtRIBA1, AtRIBA2, and AtRIBA3 in transgenic Nicotiana benthaminana chloroplasts using Agrobacterium tumefaciens pGV2260 transfection, isozymes AtRIBA2 and AtRIBA3 fail to sufficiently compensate for a lack of RIBA1 expression
gene At5g64300, phylogenetic analysis , recombinant expression of His-tagged N-terminally truncated AtRIBA1 protein in Escherichia coli, recombinant expression of GFP-tagged isozymes AtRIBA1, AtRIBA2, and AtRIBA3 in transgenic Nicotiana benthaminana chloroplasts using Agrobacterium tumefaciens pGV2260 transfection, isozymes AtRIBA2 and AtRIBA3 fail to sufficiently compensate for a lack of RIBA1 expression
gene RIB3, expression of the wild-type gene restores the ability to respire in the aE280/U1 pet mutant A137T of Saccharomyces cerevisiae, which is partially deficient in cytochromes a, a3, and cytochrome b
-
gene ribB, DNA determination and analysis, overexpression in strain M15
-
gene ribB, overexpression in an rib-defective mutant strain
gene ribB, overexpression in Escherichia coli wild-type strain RF01
gene ribB, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
LcRIBA, sequence comparisons, quantitative real-time PCR expression analysis
overexpression in strain 1012 by gene insertion in the sacB locus
-
recombinant expression of His-tagged DHBPS and GCHII domains of MtbribA2 in Escherichia coli strain BL21 (DE3)
recombinant expression of wild-type andn mutant soluble His-tagged enzymes in Escherichia coli strain BL21(DE3)
subcloning in strain DH5alpha, expression in strain BL21(DE3)
-