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Literature summary for 4.1.99.12 extracted from

  • Richter, G.; Krieger, C.; Volk, R.; Kis, K.; Ritz, H.; Gotze, E.; Bacher, A.
    Biosynthesis of riboflavin: 3,4-dihydroxy-2-butanone-4-phosphate synthase (1997), Methods Enzymol., 280, 374-382.
    View publication on PubMed
Search for more literature for 4.1.99.12

Cloned(Commentary)

Cloned (Comment) Organism
gene ribB, overexpression in an rib-defective mutant strain Escherichia coli K-12

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition Escherichia coli K-12

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli K-12

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23251
-
 x * 23251, recombinant enzyme, mass spectrometry Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate Escherichia coli K-12 the enzyme is important in the riboflavin biosynthesis, the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, is formed by condensation of 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione (1) with 3,4-dihydroxy-2-butanone 4-phosphate, overview formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12 P0A7J0 gene ribB
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion and cation exchange chromatography, hydroxyapatite chromatography, and gel filtration, to homogeneity, recombinant enzyme from the overexpressing strain by anion exchange chromatography and gel filtration Escherichia coli K-12

Reaction

Reaction Comment Organism Reaction ID
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate catalytic mechanism Escherichia coli K-12
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.283
-
 purified recombinant enzyme, assay method development, overview Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate substrate preparation, overview Escherichia coli K-12 formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
D-ribulose 5-phosphate the enzyme is important in the riboflavin biosynthesis, the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, is formed by condensation of 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione (1) with 3,4-dihydroxy-2-butanone 4-phosphate, overview Escherichia coli K-12 formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 23251, recombinant enzyme, mass spectrometry Escherichia coli K-12

Synonyms

Synonyms Comment Organism
DHBP synthase
-
 Escherichia coli K-12

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli K-12

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli K-12

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor required Escherichia coli K-12