Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a potential anti-infective target in the pathogenic yeast | Candida albicans |
Cloned (Comment) | Organism |
---|---|
functional expression of the synthetic gene in Escherichia coli strains XL1-Blue and M15 | Candida albicans |
Crystallization (Comment) | Organism |
---|---|
purified recombinant apoenzyme in complex with the substrate ribulose 5-phosphate, sitting drop vapour diffusion method, 0.003 ml of 17-34 mg/ml protein in 50 mM Tris-HCl, pH 7.5, is mixed with 0.001 ml of reservoir solution containing 85 mM sodium citrate, pH 5.0, and 17% PEG 8000, with or without 5 mM EDTA, equilibration against 0.3 ml reservoir solution, 0.003 ml of the complex solution is mixed with 0.001 ml of 90 mM Mes/NaOH, pH 6.0, containing 18% PEG 8000, addition of 2 mM D-ribulose 5-phosphate, 20°C, X-ray diffraction structure determination and analysis at 1.6-1.7 A resolution, molecular replacement, modelling | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
C59A | site-directed mutagenesis, the mutant shows 70% of wild-type enzyme activity | Candida albicans |
D92A | site-directed mutagenesis, inactive mutant | Candida albicans |
E166A | site-directed mutagenesis, inactive mutant | Candida albicans |
Q181R/Q183R | site-directed mutagenesis, construction of a synthetic gene, derived from orf 6.2440, with nucleotide exchanges at positions 414, 426, 477, 480, and 581 | Candida albicans |
Y87A | site-directed mutagenesis, the mutant shows 2% of wild-type enzyme activity | Candida albicans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not involved in catalysis, stabilize the enzyme | Candida albicans | |
Mg2+ | essential for activity | Candida albicans | |
Zn2+ | not involved in catalysis, stabilize the enzyme | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22530 | - |
2 * 22530, mass spectrometry, 2 * 22658, full-length enzyme, sequence calculation | Candida albicans |
22658 | - |
2 * 22530, mass spectrometry, 2 * 22658, full-length enzyme, sequence calculation | Candida albicans |
41000 | - |
recombinant enzyme, analytical ultracentrifugation, hydrodynamic studies | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | Candida albicans | the enzyme is involved in riboflavin biosynthesis | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | Q5A3V6 | wild-type enzyme; ATCC 10231D, orf 6.2440 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, expressed from the synthetic gene in Escherichia coli, to homogeneity by hydrophobic interaction chromatography, ultrafiltration, and gel filtration | Candida albicans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate | reaction mechanism, the enzyme possesses an essential acidic active-site loop | Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.332 | - |
purified recombinant enzyme | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | - |
Candida albicans | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
D-ribulose 5-phosphate | the enzyme is involved in riboflavin biosynthesis | Candida albicans | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 22530, mass spectrometry, 2 * 22658, full-length enzyme, sequence calculation | Candida albicans |
More | the enzyme possesses an essential acidic active-site loop, active site structure, structure comparisons, overview | Candida albicans |
Synonyms | Comment | Organism |
---|---|---|
3,4-dihydroxy-2-butanone 4-phosphate synthase | - |
Candida albicans |
DHBPS | - |
Candida albicans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Candida albicans |