Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target in antimicrobial inhibitor development, structure-based inhibitor design | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
subcloning in strain DH5alpha, expression in strain BL21(DE3) | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, cannot be substituted by Ca2+, KM: 0.99 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23177 | - |
x * 23177, mass spectrometry | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | Escherichia coli | step in biosynthesis of vitamin B2, riboflavin | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
D-ribulose 5-phosphate | Escherichia coli W3110 / ATCC 27325 | step in biosynthesis of vitamin B2, riboflavin | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli W3110 / ATCC 27325 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from strain Bl21(DE3) by hydrophobic interaction and anion exchange chromatography, and ultrafiltration | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
development of a spectrophotometric/colorimetric assay method, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | - |
Escherichia coli | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
D-ribulose 5-phosphate | step in biosynthesis of vitamin B2, riboflavin | Escherichia coli | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
D-ribulose 5-phosphate | - |
Escherichia coli W3110 / ATCC 27325 | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
D-ribulose 5-phosphate | step in biosynthesis of vitamin B2, riboflavin | Escherichia coli W3110 / ATCC 27325 | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23177, mass spectrometry | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |