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Literature summary for 4.1.99.12 extracted from

  • Richter, G.; Kelly, M.; Krieger, C.; Yu, Y.; Bermel, W.; Karlsson, G.; Bacher, A.; Oschkinat, H.
    NMR studies on the 46-kDa dimeric protein, 3,4-dihydroxy-2-butanone 4-phosphate synthase, using 2H, 13C, and 15N-labelling (1999), Eur. J. Biochem., 261, 57-65.
    View publication on PubMed
Search for more literature for 4.1.99.12

Cloned(Commentary)

Cloned (Comment) Organism
gene ribB, DNA determination and analysis, overexpression in strain M15 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 2 * 23000, SDS-PAGE, hydrodynamic analysis, NMR structure study, 2 * 23351, mass spectrometry Escherichia coli
23351
-
 2 * 23000, SDS-PAGE, hydrodynamic analysis, NMR structure study, 2 * 23351, mass spectrometry Escherichia coli
44800
-
 recombinant enzyme, analytical ultracentrifugation at 37°C Escherichia coli
46300
-
 recombinant enzyme, hydrodynamic analysis, NMR structure study, analytical ultracentrifugation at 4°C, overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate Escherichia coli the enzyme is involved in the pathway of riboflavin biosynthesis formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene ribB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from strain M15 by anion exchange chromatography and gel filtration Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate catalytic mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.152
-
 purified recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate
-
 Escherichia coli formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
D-ribulose 5-phosphate the enzyme is involved in the pathway of riboflavin biosynthesis Escherichia coli formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23000, SDS-PAGE, hydrodynamic analysis, NMR structure study, 2 * 23351, mass spectrometry Escherichia coli
More hydrodynamic analysis, NMR structure study, isotope labeling, the homodimeric protein obeys strict C2 symmetry, overview Escherichia coli

Synonyms

Synonyms Comment Organism
3,4-dihydroxy-2-butanone 4-phosphate synthase
-
 Escherichia coli