4.1.2.47: (S)-hydroxynitrile lyase
This is an abbreviated version!
For detailed information about (S)-hydroxynitrile lyase, go to the full flat file.
Word Map on EC 4.1.2.47
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4.1.2.47
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esculenta
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manihot
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brasiliensis
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hevea
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synthesis
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cassava
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ketone
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hcn
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lyases
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benzaldehyde
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s-mandelonitrile
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rubber
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athnl
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crantz
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hydrocyanic
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s-cyanohydrins
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r-selective
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arylacetonitrilase
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s-enantiomer
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cyanogenesis
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montanum
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s-mandelic
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pharmacology
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agriculture
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analysis
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drug development
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food industry
- 4.1.2.47
- esculenta
- manihot
- brasiliensis
- hevea
- synthesis
- cassava
- ketone
- hcn
- lyases
- benzaldehyde
-
s-mandelonitrile
- rubber
- athnl
- crantz
-
hydrocyanic
-
s-cyanohydrins
-
r-selective
- arylacetonitrilase
-
s-enantiomer
-
cyanogenesis
- montanum
-
s-mandelic
- pharmacology
- agriculture
- analysis
- drug development
- food industry
Reaction
Synonyms
(R)-LuHNL, (R)-Oxynitrilase, (S)-cyanohydrin producing hydroxynitrile lyase, (S)-Hb-HNL, (S)-HbHNL, (S)-HNL, (S)-Hydroxynitrile lyase, (S)-Me-HNL, (S)-MeHNL, (S)-Oxynitrilase, (S)-selective HNL, (S)-selective hydroxynitrile lyase, Acetone cyanohydrin lyase, ACL, alpha-Hydroxynitrile lyase, EC 4.1.2.37, EC 4.1.2.39, Hb-HNL, HbHNL, HNL, HNL1, Hydroxynitrile lyase, LuHNL, MeHNKL, MeHNL, mut-HNL1, Oxynitrilase, S-HnL, S-hydroxynitrile lyase, S-selective HnL, S-selective hydroxynitrile lyase, S-stereoselective HNL
ECTree
4.1.2.47 (S)-hydroxynitrile lyaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 4.1.2.47 - (S)-hydroxynitrile lyase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
0 - 60
the enzyme is stable and exhibits more than 96 h half-life time between 0 and 20°C, the enzyme shows more than 48 h half-life time at 30°C, 64 h half-life time at 37°C, 2.7 h half-life time at 50°C, and 0.5 h half-life time at 60°C
30
half-life: 1755 min in phosphate buffer, 1379 min in glutamate buffer, 2315 min in citrate buffer
40
half-life: 690 min in phosphate buffer, 204 min in glutamate buffer, 322 min in citrate buffer
40 - 50
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the enzyme shows a half-life time of 411 h at 40°C. At 50°C a rapid inactivation is observed
45
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mutant enzyme and wild-type enzyme G113S are stable for 20 min at pH 5.4
50
60
65
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20 min at pH 5.4, mutant enzyme G113S loses about 15% of its initial activity, wild-type enzyme loses about 50% of its initial activity
70
additional information
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the enzyme is much more thermostable in 1-propyl-3-methylimidazolium tetrahydroborate than in acetonitrile or tetrahydrofuran
50
half-life: 342 min in phosphate buffer, 80 min in glutamate buffer, 115 min in citrate buffer
60
half-life: 56 min in phosphate buffer, 42 min in glutamate buffer, 29 min in citrate buffer
60
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20 min at pH 5.4, mutant enzyme G113S loses about 10% of its initial activity, wild-type enzyme loses about 20% of its initial activity. More than 50% loss of activity of wild-type enzyme after 50 min, 10% loss of activity of mutant enzyme G113 after 50 min, 75% loss of activity of wild-type enzyme after 120 min, 25% loss of activity of mutant enzyme G113 after 120 min
70
half-life: 10 min in phosphate buffer, 15 min in glutamate buffer, 7 min in citrate buffer
70
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20 min at pH 5.4, about 80% loss of activity, mutant enzyme G113S and wild-type enzyme
