4.1.2.47: (S)-hydroxynitrile lyase

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For detailed information about (S)-hydroxynitrile lyase, go to the full flat file.

Word Map on EC 4.1.2.47

4.1.2.47

esculenta

manihot

brasiliensis

hevea

synthesis

cassava

ketone

hcn

lyases

benzaldehyde

s-mandelonitrile

rubber

athnl

crantz

hydrocyanic

s-cyanohydrins

r-selective

arylacetonitrilase

s-enantiomer

cyanogenesis

montanum

s-mandelic

pharmacology

agriculture

analysis

drug development

food industry

Reaction

Synonyms

(R)-LuHNL, (R)-Oxynitrilase, (S)-cyanohydrin producing hydroxynitrile lyase, (S)-Hb-HNL, (S)-HbHNL, (S)-HNL, (S)-Hydroxynitrile lyase, (S)-Me-HNL, (S)-MeHNL, (S)-Oxynitrilase, (S)-selective HNL, (S)-selective hydroxynitrile lyase, Acetone cyanohydrin lyase, ACL, alpha-Hydroxynitrile lyase, EC 4.1.2.37, EC 4.1.2.39, Hb-HNL, HbHNL, HNL, HNL1, Hydroxynitrile lyase, LuHNL, MeHNKL, MeHNL, mut-HNL1, Oxynitrilase, S-HnL, S-hydroxynitrile lyase, S-selective HnL, S-selective hydroxynitrile lyase, S-stereoselective HNL

ECTree

4 Lyases

4.1 Carbon-carbon lyases

4.1.2 Aldehyde-lyases

4.1.2.47 (S)-hydroxynitrile lyase

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Results	in table
3	Activating Compound
3	AA Sequence
27	Application
33	Cloned(Commentary)
3	Cofactor
16	Crystallization (Commentary)
2	Expression
1	General Information
8	General Stability
50	Inhibitors
77	kcat/KM [mM/s]
2	Ki Value [mM]
135	KM Value [mM]
2	Localization
23	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
4	Organic Solvent Stability
94	Organism
2	Oxidation Stability
3	Pathway
12	PDB ID
23	pH Optimum
4	pH Range
17	pH Stability
2	pI Value
2	Posttranslational Modification
99	Protein Variants
26	Purification (Commentary)
3	Reaction
6	Reaction Type
91	Reference
20	Source Tissue
76	Specific Activity [micromol/min/mg]
1	Storage Stability
368	Substrates and Products (Substrate)
14	Subunits
134	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
3	Temperature Range [°C]
17	Temperature Stability [°C]
100	Turnover Number [1/s]

Purification

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Purification on EC 4.1.2.47 - (S)-hydroxynitrile lyase

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10 entries

mutant enzymes Hnl-E79A, Hnl-S80A, Hnl-C81S, and Hnl-H235A and the wild-type protein are purified to homogeneity, and Hnl-H10A is partially purified, by ion exchange chromatography and native polyacrylamide gel electrophoresis

Hevea brasiliensis

P52704

706644

mutant K236L, recombinant

Hevea brasiliensis

P52704

662210

Ni-NTA agarose column chromatography and Sephadex G-75 gel filtration

partial

preparation of a cross-linked enzyme aggregate (CLEA) from hydroxynitrile lyase from Hevea brasiliensis, precipitated hydroxynitrile lyase, comparison of free, cross-linked enzyme aggregate (CLEA) and immobilized in sol-gel (aqua gel)

Hevea brasiliensis

671190

Q-Sepharose column chromatography

recombinant enzyme

wild-type and W128A mutant partially purified

Manihot esculenta

P52705

691345