Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Baliospermum montanum |
Protein Variants | Comment | Organism |
---|---|---|
D207A | mutant protein is insoluble | Baliospermum montanum |
H235A | mutant protein is insoluble | Baliospermum montanum |
additional information | C-terminally truncated enzymes exhibit activities of 75, 102, 65 and 48 U/l for enzyme after removing 2, 4, 6, and 8 amino acids, respectively | Baliospermum montanum |
S80A | mutant protein is insoluble | Baliospermum montanum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 12% residual activity | Baliospermum montanum | |
methyl ethyl ketone | 50 mM, 27% residual activity | Baliospermum montanum | |
phenol | 50 mM, 32% residual activity | Baliospermum montanum | |
Phenylmethyl sulfonylfluoride | 1 mM, 27% residual activity | Baliospermum montanum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
2,4-dimethylbenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
5.5 | - |
benzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
9.8 | - |
3-Methoxybenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
14.4 | - |
3-methylbenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
15.7 | - |
piperonal | pH 6.0, 25°C | Baliospermum montanum | |
21.7 | - |
2-Methylbenzaldehyde | pH 6.0, 25°C | Baliospermum montanum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29500 | - |
2 * 29500, SDS-PAGE | Baliospermum montanum |
60000 | 65000 | gel filtration | Baliospermum montanum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Baliospermum montanum | D1MX73 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Baliospermum montanum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
178 | - |
pH 6.0, 25°C | Baliospermum montanum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanide + 2,4-dimethylbenzaldehyde | - |
Baliospermum montanum | (2S)-(2,4-dimethylphenyl)(hydroxy)ethanenitrile | 65% yield, 82% enantiomeric excess | ? | |
cyanide + 2-methylbenzaldehyde | - |
Baliospermum montanum | (2S)-(2-methylphenyl)(hydroxy)ethanenitrile | 76% yield, 47% enantiomeric excess | ? | |
cyanide + 3-methoxybenzaldehyde | - |
Baliospermum montanum | (2S)-(3-methoxyphenyl)(hydroxy)ethanenitrile | 67% yield, 76% enantiomeric excess | ? | |
cyanide + 3-methylbenzaldehyde | - |
Baliospermum montanum | (2S)-(3-methylphenyl)(hydroxy)ethanenitrile | 76% yield, 76% enantiomeric excess | ? | |
cyanide + benzaldehyde | - |
Baliospermum montanum | (S)-mandelonitrile | 68% yield, 54% enantiomeric excess | ? | |
cyanide + piperonal | - |
Baliospermum montanum | ? | 47% yield, 74% enantiomeric excess | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 29500, SDS-PAGE | Baliospermum montanum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Baliospermum montanum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
65 | - |
Baliospermum montanum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.5 | - |
2,4-dimethylbenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
26.6 | - |
benzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
28.1 | - |
3-Methoxybenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
49.2 | - |
3-methylbenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
53.6 | - |
2-Methylbenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
91.4 | - |
piperonal | pH 6.0, 25°C | Baliospermum montanum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Baliospermum montanum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | - |
Baliospermum montanum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.4 | 11 | - |
Baliospermum montanum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.47 | - |
2-Methylbenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
2.87 | - |
3-Methoxybenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
3.41 | - |
3-methylbenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
3.56 | - |
2,4-dimethylbenzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
4.8 | - |
benzaldehyde | pH 6.0, 25°C | Baliospermum montanum | |
5.82 | - |
piperonal | pH 6.0, 25°C | Baliospermum montanum |